AtpH 抗体
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北京 101111
Quick Overview for AtpH 抗体 (ABIN249344)
抗原
适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-AtpH | ATP synthase subunit c (chloroplastic)
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特异性
- The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthasesubcomplex, as well as the chloroplastic CF1 ATP synthase and most knownbacterial F-type ATP synthases.
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交叉反应 (详细)
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Arabidopsis thaliana, Nicotiana benthamina, Spinacia oleracea, Thermosynechococcus elongatus
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
预测反应
- Algae,Cannabis sativa,Cyclotella cryptica,Glycine max,Oryza sativa,Physcomitrium patens,Phaeodactylum tricornutum,Pisum sativum,Populus alba,Pinus thunbergii,Thalassiosira pseudonana,Zea mays,Vitis vinifera
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纯化方法
- Serum
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免疫原
- Purified c subunit from Spinacia oleracea UniProt: P69447.
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应用备注
- 1 : 1000-1 : 10 000 (WB)
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说明
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please note that increased incubation at 95°C (20-30 min) prior to loading is recommended to break the multimeric c-mer structure, detection of partial ring structures (e.g. 5 or 6 subunits) may occur
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 200 μL of sterile water
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缓冲液
- Serum
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- AtpH
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背景
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Background: F-type ATPase (ATP synthase) is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient. Multiple copies of the c subunit build up the ring structure (in spinach a 14-mer of ~112 kDa) of the membrane bound Fo-part of the enzyme.
Additional information: This product can be sold containing proclin if requested.
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分子量
- 8 kDa (for Arabidopsis thaliana)
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基因ID
- 2715579
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UniProt
- P69447
抗原
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