XTH-Xet 抗体
There is 1 publication for this product available. The 兔 多克隆 anti-XTH-Xet antibody is suitable to detect XTH-Xet in samples from Barley 和 Oryza sativa. It has been validated for ELISA 和 WB.
(1 reference)
产品编号 ABIN249338
发货至:
中国
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Quick Overview for XTH-Xet 抗体 (ABIN249338)
抗原
XTH-Xet
适用
Barley, Oryza sativa
宿主
兔
克隆类型
多克隆
应用范围
ELISA, Western Blotting (WB)
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原理
- Anti-XTH-Xet | XET5 Xyloglucan xyloglucosyl transferase
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交叉反应 （详细）
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Hordeum vulgare, Oryza sativa
Not reactive in: Poplar sp., Zea mays -
纯化方法
- Serum
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免疫原
- Two synthetic peptides from highly conserved region of Horderum vulgare XTH-Xet
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应用备注
- 1 : 5000 (ELISA), 1 : 500 (WB)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 100 μL of sterile water
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缓冲液
- Serum
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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: "A Barley xyloglucan xyloglucosyl transferase covalently links xyloglucan, cellulosic substrates, and (1,3;1,4)-beta-D-glucans." in: The Journal of biological chemistry, Vol. 282, Issue 17, pp. 12951-62, (2007) (PubMed).
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: "A Barley xyloglucan xyloglucosyl transferase covalently links xyloglucan, cellulosic substrates, and (1,3;1,4)-beta-D-glucans." in: The Journal of biological chemistry, Vol. 282, Issue 17, pp. 12951-62, (2007) (PubMed).
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- XTH-Xet
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背景
- Molecular interactions between wall polysaccharides, which include cellulose and a range of non-cellulosic polysaccharides such as xyloglucans and (1,3,1,4)-?-d-glucans, are fundamental to cell wall properties. These interactions have been assumed to be non-covalent in nature in most cases. A highly purified barley xyloglucan xyloglucosyl transferase HvXET5 (EC 2.4.1.207), a member of the GH16 group of glycoside hydrolases, catalyses the in vitro formation of covalent linkages between xyloglucans and cellulosic substrates, and between xyloglucans and (1,3,1,4)-?-d-glucans. It is possible that XETs could link different polysaccharides in vivo, and hence influence cell wall strength, flexibility and porosity.
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分子量
- 31.5 | 33 kDa
抗原
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