XTH-Xet 抗体
This anti- antibody is a 兔 多克隆 antibody detecting in ELISA 和 WB. Suitable for Barley 和 Oryza sativa. This Primary Antibody has been cited in 1 publication.
(1 reference)Quick Overview for XTH-Xet 抗体 (ABIN249338)
抗原
XTH-Xet
适用
Barley, Oryza sativa
宿主
兔
克隆类型
多克隆
应用范围
ELISA, Western Blotting (WB)
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交叉反应 （详细）
- Not reactive in: Zea mays
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预测反应
- monocots
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产品特性
- Expected / apparent Molecular Weight of the Antigene: 31.5 / 33 kDa
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纯化方法
- serum
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免疫原
- two synthetic peptides from highly conserved region of Horderum vulgare XTH-Xet
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应用备注
- 1: 500 with standard ECL (WB) , 1: 5000 (ELISA)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 100 μL of sterile water.
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注意事项
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. -
储存条件
- -20 °C
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储存方法
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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: "A Barley xyloglucan xyloglucosyl transferase covalently links xyloglucan, cellulosic substrates, and (1,3;1,4)-beta-D-glucans." in: The Journal of biological chemistry, Vol. 282, Issue 17, pp. 12951-62, (2007) (PubMed).
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: "A Barley xyloglucan xyloglucosyl transferase covalently links xyloglucan, cellulosic substrates, and (1,3;1,4)-beta-D-glucans." in: The Journal of biological chemistry, Vol. 282, Issue 17, pp. 12951-62, (2007) (PubMed).
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- XTH-Xet
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背景
- Molecular interactions between wall polysaccharides, which include cellulose and a range of non-cellulosic polysaccharides such as xyloglucans and (1,3,1,4)-β-d-glucans, are fundamental to cell wall properties. These interactions have been assumed to be non-covalent in nature in most cases. A highly purified barley xyloglucan xyloglucosyl transferase HvXET5 (EC 2.4.1.207), a member of the GH16 group of glycoside hydrolases, catalyses the in vitro formation of covalent linkages between xyloglucans and cellulosic substrates, and between xyloglucans and (1,3,1,4)-β-d-glucans. It is possible that XETs could link different polysaccharides in vivo, and hence influence cell wall strength, flexibility and porosity.
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分子量
- expected: 31.5 kDa, apparent: 33 kDa
抗原
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