Xylose 抗体
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北京 101111
Quick Overview for Xylose 抗体 (ABIN249329)
抗原
适用
宿主
克隆类型
应用范围
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原理
- Anti-Xylose
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特异性
- Antibody binds to heavy chains of Goat IgG and light chains of all Goat immunoglobuli
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交叉反应 （详细）
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Higher plants and algae
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
预测反应
- Higher plants
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纯化方法
- Immunogen affinity purified serum
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免疫原
- xylose residues bound to the N-glycan in beta 1
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应用备注
- 1 μg/mL (ELISA), 2 μg/10 mL incubation buffer (WB)
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说明
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negative control: Fetuin, a glycoprotein containing fucose linked in alpha 1.6 and no xylose, Sigma, product number F3385.positive control: Type II - horseradish peroxidase which contains β1.2 Xylose and α1.3 fucose, Sigma, product number P8250
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL of sterile water
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缓冲液
- Immunogen affinity purified serum in PBS pH 7.4.
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- Xylose
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背景
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Background: This antibody specifically cross-reacts against xylose residues bound to the protein N-glycans in beta1,2. This residue is characterisitc of the plant protein N-glycans and is absent in protein N-glycans from animals. This residue is added in the Golgi apparatus.
Additional information: Beta (1,2) xylose is present exclusively in plant N-glycans so antibodies against this sugar moiety should not cross-react with any mammal glycoprotein.This antibody do not bind free D-xylose. This antibody does not seem to work in immunolocalization.
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分子量
- 10-100 for various glycoproteins
抗原
-
