P4HB 抗体 (AA 409-509) (Atto 390)
Quick Overview for P4HB 抗体 (AA 409-509) (Atto 390) (ABIN2486765)
抗原
See all P4HB 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 409-509
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特异性
- Detects ~58 kDa.
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交叉反应
- Cow, 犬, 豚鼠, Hamster, 人, Mollusca, 小鼠, Pig, 大鼠, 绵羊, 非洲爪蟾
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纯化方法
- Peptide Affinity Purified
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免疫原
- Rat PDI synthetic peptide (409-509 aa C-terminal) conjugated to KLH
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anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 1-508) antibody
P4HB 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) antibodyP4HB 适用: 人 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) antibodyP4HB 适用: 人 WB, IF, ICC, IHC (p), IEM 宿主: 兔 Polyclonal unconjugated
anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (N-Term) antibodyP4HB 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 兔, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 329-508) antibodyP4HB 适用: 人 WB, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 1-508) antibodyP4HB 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 409-509) antibodyP4HB 适用: 大鼠 WB, IHC, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (C-Term) antibodyP4HB 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, Pig, 兔, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 409-509) antibody (FITC)P4HB 适用: 大鼠 WB, IHC, IP, IF, ICC 宿主: 兔 Polyclonal FITC
anti-Prolyl 4-Hydroxylase, beta Polypeptide (P4HB) (AA 409-509) antibody (Biotin)P4HB 适用: 大鼠 WB, IHC, IP, IF, ICC 宿主: 兔 Polyclonal Biotin
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应用备注
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- WB (1:1000)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
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说明
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A 1:1000 dilution of ABIN2486765 was sufficient for detection of PDI in 20 μg of HeLa cell lysate by ECL immunoblot analysis.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Conjugated antibodies should be stored at 4°C
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- P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))
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别名
- PDI
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背景
- The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation via its oxidase activity and isomerization via its isomerase activity, as well as the reduction of disulphide bonds in proteins (1). Studies suggest BiP and PDI work together sequentially to increase oxidation of these proteins (2, 3). PDI has also been found to function as a chaperone to prevent the aggregation of unfolded substrates, and serves as a subunit of prolyl 4-hydroxylase and microsomal triglyceride transferase (4, 5). PDI is an abundant 55 kDa protein located primarily in the ER, however studies have also proved its presence in the cytosol (1). PDI has the ability to reside in the ER permanently due to the highly conserved KDEL sequence at its carboxy-terminus (6). It uses carboxy-terminal KDEL as a retention signal, and this appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor (7).
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基因ID
- 287164
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NCBI登录号
- NP_001099245
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UniProt
- P04785
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途径
- Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism
抗原
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