CENPA 抗体 (APC)
Quick Overview for CENPA 抗体 (APC) (ABIN2486494)
抗原
See all CENPA 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Detects ~18 kDa. Recognizes Human CENP-A.
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交叉反应
- 人
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纯化方法
- Protein G Purified
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免疫原
- Synthetic peptide corresponding to a portion of human CENP-A
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亚型
- IgG1
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anti-Centromere Protein A (CENPA) (pSer16), (pSer18) antibody
CENPA 适用: 人 WB, IF, ICC, DB 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein A (CENPA) (N-Term) antibodyCENPA 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein A (CENPA) (AA 1-100) antibodyCENPA 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Centromere Protein A (CENPA) antibodyCENPA 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal 5A7-2E11 unconjugated
anti-Centromere Protein A (CENPA) antibody (HRP)CENPA 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal 5A7-2E11 HRP
anti-Centromere Protein A (CENPA) antibody (FITC)CENPA 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal 5A7-2E11 FITC
anti-Centromere Protein A (CENPA) antibody (Biotin)CENPA 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal 5A7-2E11 Biotin
anti-Centromere Protein A (CENPA) antibody (PE)CENPA 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal 5A7-2E11 PE
anti-Centromere Protein A (CENPA) antibody (PerCP)CENPA 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal 5A7-2E11 PerCP
anti-Centromere Protein A (CENPA) antibody (Atto 488)CENPA 适用: 人 WB, IF, ICC 宿主: 小鼠 Monoclonal 5A7-2E11 Atto 488
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应用备注
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- WB (1:1000)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
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说明
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1 μg/ml of ABIN2486494 was sufficient for detection of CENPA in 20 μg of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Conjugated antibodies should be stored at 4°C
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- CENPA (Centromere Protein A (CENPA))
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别名
- CENPA
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背景
- A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The Centromere Protein-A, CENP-A, is a Histone H3-like protein that contains a C-terminal H3-like domain, which is required for centromere localization of CENP-A, and an antigenic N-terminal domain. CENP-A, originally isolated from HeLa cells, is essential for kinetochore targeting of CENP-C. In the presence of DNA CENP-A forms an octa-meric complex with histones H4, H2A, H2B. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes, on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on the chromosome.
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基因ID
- 1058
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NCBI登录号
- NP_001035891
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UniProt
- P49450
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途径
- Chromatin Binding, Maintenance of Protein Location
抗原
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