Phosphoserine 抗体 (Atto 594)
Quick Overview for Phosphoserine 抗体 (Atto 594) (ABIN2486253)
抗原
适用
宿主
克隆类型
标记
应用范围
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特异性
- Detects proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine.
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纯化方法
- Peptide Affinity Purified
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免疫原
- Phosphoserine conjugated to KLH, and phosvitin mixture
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anti-Phosphoserine antibody
WB, ELISA, IHC 宿主: 小鼠 Monoclonal unconjugated
anti-Phosphoserine antibody适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal 7A3 unconjugated
anti-Phosphoserine (phosphorylated) antibodyWB 宿主: 小鼠 Monoclonal 5B12 unconjugated
anti-Phosphoserine antibodyWB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Phosphoserine antibody (FITC)WB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal FITC
anti-Phosphoserine antibody (Biotin)WB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal Biotin
anti-Phosphoserine antibody (Atto 488)WB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal Atto 488
anti-Phosphoserine antibody (Atto 390)WB, ELISA, IP, IHC, ICC, IF 宿主: 兔 Polyclonal Atto 390
anti-Phosphoserine antibody适用: 小鼠 WB, ELISA, IP, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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- WB (1:500)
- ICC/IF (1:50)
- ELISA (1:250)
- IP (1:100)
- optimal dilutions for assays should be determined by the user.
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说明
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2 μg/ml of ABIN2486253 was sufficient for detection of phosphorylation signal in western blot analysis using human MMRU cells treated with 0.1 μM okadaic acid.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS, 50 % glycerol, 0.01 % sodium azide, Storage buffer may change when conjugated
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Conjugated antibodies should be stored at 4°C
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- Phosphoserine
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物质类
- Amino Acid
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背景
- Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine, others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).
抗原
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