RAB5 抗体 (PerCP)
Quick Overview for RAB5 抗体 (PerCP) (ABIN2486179)
抗原
See all RAB5 (RAB5A) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Detects ~26 kDa.
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交叉反应
- Cow, 人, 猴, 小鼠, 大鼠
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纯化方法
- Protein A Purified
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免疫原
- Human Rab5 synthetic peptide conjugated to KLH, identical to dog Rab5 sequence over the residues
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anti-RAB5A, Member RAS Oncogene Family (RAB5A) (C-Term) antibody
RAB5A 适用: 人, 小鼠, 大鼠, Cow, 猴, 犬, 绵羊, 马, 兔, 豚鼠, Pig, Hamster, 小鸡, Avian, Cat, 驴, 山羊 WB, IF, IHC (p), IHC (fro) 宿主: 山羊 Polyclonal unconjugated
anti-RAB5A, Member RAS Oncogene Family (RAB5A) (C-Term) antibodyRAB5A 适用: 人, 小鼠, 大鼠, Cow, 猴, 犬, 绵羊, 马, 兔, 豚鼠, Pig, Hamster, 小鸡, Avian, Cat, 驴, 山羊 WB, IF 宿主: 山羊 Polyclonal unconjugated
anti-RAB5A, Member RAS Oncogene Family (RAB5A) antibodyRAB5A 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-RAB5A, Member RAS Oncogene Family (RAB5A) (AA 1-215) antibodyRAB5A 适用: 人 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 3H5C11 unconjugated
anti-RAB5A, Member RAS Oncogene Family (RAB5A) (AA 1-215) antibodyRAB5A 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-RAB5A, Member RAS Oncogene Family (RAB5A) antibody (Atto 390)RAB5A 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal Atto 390
anti-RAB5A, Member RAS Oncogene Family (RAB5A) (AA 182-211) antibodyRAB5A 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-RAB5A, Member RAS Oncogene Family (RAB5A) antibody (HRP)RAB5A 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal HRP
anti-RAB5A, Member RAS Oncogene Family (RAB5A) antibody (FITC)RAB5A 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal FITC
anti-RAB5A, Member RAS Oncogene Family (RAB5A) antibody (Biotin)RAB5A 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal Biotin
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应用备注
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- WB (1:1000)
- IHC (1:100)
- ICC/IF (1:80)
- optimal dilutions for assays should be determined by the user.
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说明
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1 μl/ml of ABIN2486179 was sufficient for detection of Rab5 in 15 μg of HeLa lysate by ECL immunoblot analysis using Donkey anti-rabbit IgG:HRP as the secondary antibody.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Conjugated antibodies should be stored at 4°C
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- RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))
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别名
- Rab5
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背景
- Rab5 is a 24 kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound inactive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs) (1, 2). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a subcellular compartment (3). Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion(1). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hyper variable COHO-terminal domains with a cysteine motif implicated in subcellular targeting. Post-translational modification of the cysteine motif with one or two geranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins(3). Each Rab shows a characteristic subcellular distribution (4). In particular, Rab5 is ubiquitously expressed in human tissues. It localizes mainly to early endosomes, but is also present on the plasma membrane. It regulates the fusion between endocytic vesicles and early endosomes, as well as the homotypic fusion between early endosomes (5). Among the proteins recruited by the GTP-bound active Rab5 are Rabaptin-5 and EEA1 (6). Anti-Rab5 may be used as an early endosome marker.
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基因ID
- 5868
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NCBI登录号
- NP_004153
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UniProt
- P20339
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途径
- Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity
抗原
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