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RAB5 抗体 (PerCP)

This anti-RAB5 antibody is a Rabbit Polyclonal antibody detecting RAB5 in WB, IHC, IF, ICC. Suitable for Human.
产品编号 ABIN2486179
发货至: 中国

Quick Overview for RAB5 抗体 (PerCP) (ABIN2486179)

抗原

See all RAB5 (RAB5A) 抗体
RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

适用

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宿主

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克隆类型

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多克隆

标记

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This RAB5 antibody is conjugated to PerCP

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
  • 特异性

    Detects ~26 kDa.

    交叉反应

    Cow, 人, 猴, 小鼠, 大鼠

    纯化方法

    Protein A Purified

    免疫原

    Human Rab5 synthetic peptide conjugated to KLH, identical to dog Rab5 sequence over the residues
  • 应用备注

    • WB (1:1000)
    • IHC (1:100)
    • ICC/IF (1:80)
    • optimal dilutions for assays should be determined by the user.

    说明

    1 μl/ml of ABIN2486179 was sufficient for detection of Rab5 in 15 μg of HeLa lysate by ECL immunoblot analysis using Donkey anti-rabbit IgG:HRP as the secondary antibody.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Conjugated antibodies should be stored at 4°C
  • 抗原

    RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

    别名

    Rab5

    背景

    Rab5 is a 24 kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound inactive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs) (1, 2). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a subcellular compartment (3). Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion(1). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hyper variable COHO-terminal domains with a cysteine motif implicated in subcellular targeting. Post-translational modification of the cysteine motif with one or two geranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins(3). Each Rab shows a characteristic subcellular distribution (4). In particular, Rab5 is ubiquitously expressed in human tissues. It localizes mainly to early endosomes, but is also present on the plasma membrane. It regulates the fusion between endocytic vesicles and early endosomes, as well as the homotypic fusion between early endosomes (5). Among the proteins recruited by the GTP-bound active Rab5 are Rabaptin-5 and EEA1 (6). Anti-Rab5 may be used as an early endosome marker.

    基因ID

    5868

    NCBI登录号

    NP_004153

    UniProt

    P20339

    途径

    Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity
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