DNA/RNA Damage 抗体 (PerCP)
Quick Overview for DNA/RNA Damage 抗体 (PerCP) (ABIN2486061)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes markers of oxidative damage to DNA (8-hydroxy-2'-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine).
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纯化方法
- Protein G Purified
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免疫原
- 8-hydroxy-guanosine-BSA and -casein conjugates
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亚型
- IgG2b
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anti-DNA/RNA Damage antibody (HRP)
适用: 所有种类 DB, ELISA, FACS, Func, ICC, IF, IHC, IP 宿主: 小鼠 Monoclonal 15A3 HRP
anti-DNA/RNA Damage antibody (FITC)适用: 所有种类 DB, ELISA, FACS, Func, ICC, IF, IHC, IP 宿主: 小鼠 Monoclonal 15A3 FITC
anti-DNA/RNA Damage antibody (Biotin)适用: 所有种类 DB, ELISA, FACS, Func, ICC, IF, IHC, IP 宿主: 小鼠 Monoclonal 15A3 Biotin
anti-DNA/RNA Damage antibody (PE)适用: 所有种类 DB, ELISA, FACS, Func, ICC, IF, IHC, IP 宿主: 小鼠 Monoclonal 15A3 PE
anti-DNA/RNA Damage antibody (APC)适用: 所有种类 DB, ELISA, FACS, Func, ICC, IF, IHC, IP 宿主: 小鼠 Monoclonal 15A3 APC
anti-DNA/RNA Damage antibody (Atto 488)适用: 所有种类 DB, ELISA, FACS, Func, ICC, IF, IHC, IP 宿主: 小鼠 Monoclonal 15A3 Atto 488
anti-DNA/RNA Damage antibody (Atto 594)适用: 所有种类 DB, ELISA, FACS, Func, ICC, IF, IHC, IP 宿主: 小鼠 Monoclonal 15A3 Atto 594
anti-DNA/RNA Damage antibody (Atto 390)适用: 所有种类 DB, ELISA, FACS, Func, ICC, IF, IHC, IP 宿主: 小鼠 Monoclonal 15A3 Atto 390
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应用备注
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- IHC (1:1000)
- optimal dilutions for assays should be determined by the user.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS, 50 % glycerol, 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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- DNA/RNA Damage
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物质类
- Chemical
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背景
- DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are oxidation, alkylation, hydrolysis and mismatch of the bases. During the oxidation of bases, highly reactive chemical entities collectively known as RONS, occurs. RONS stands for reactive oxygen and nitrogen species and includes nitric oxide, superoxide, hydroxyl radical, hydrogen peroxide and peroxynitrite. Numerous studies have shown that RONS causes a variety of issues including DNA damage(1). 8-hydroxyguanine, 8-hydroxy-2'-deoxyguanonsine and 8- hydroxyguanosine are all RNA and DNA markers of oxidative damage. 8-hydroxy-2'-guanosine is produced by reactive oxygen and nitrogen species including hydroxyl radical and peroxynitrite. Specifically its high biological relevance is due to its ability to induce G to T transversions, which is one of the most frequent somatic mutations (2). 8-hydroxy-guanine has been the most frequently studied type of DNA base damage, with studies in diabetes, and cancer. Base modifications of this type arise from radical-induced hydroxylation and cleavage reactions of the purine ring (3, 4). And finally, 8-hydroxy-guanosine, like 8-hydroxy-2'-guanosine, induces a mutagenic transversion of G to T in DNA. Its role has specifically been tested in the development of diabetes, hypertension and strokes (5, 6, and 7).
抗原
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