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HSP70 抗体

Name: HSP70 抗体
Brand: antibodies-online
SKU: ABIN2485875
Availability: OutOfStock

This anti-HSP70 antibody is a 大鼠单克隆 antibody detecting HSP70 in WB, ELISA, IF 和 ICC. Suitable for Drosophila melanogaster. This Primary Antibody has been cited in 1 publication.

(1 reference)

产品编号 ABIN2485875

发货至: 中国

Quick Overview for HSP70 抗体 (ABIN2485875)

抗原

See all HSP70 抗体

HSP70 (Heat Shock Protein 70 (HSP70))

适用

Drosophila melanogaster

宿主

大鼠

克隆类型

单克隆

标记

This HSP70 antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

克隆位点

7FB

Discover our top product HSP70 Primary Antibody

特异性

Detects ~70 kDa (heat-inducible form).

交叉反应

Drosophila melanogaster

纯化方法

Protein G Purified

免疫原

Prepared from Drosophila tissue culture cells heat shocked at 36.5C for 3 hours, and isolated using SDS PAGE.

亚型

IgG2b
anti-Heat Shock Protein 70 (HSP70) antibody
HSP70 适用: 人 WB, IHC, ELISA, IF, ICC, FACS, IEM, AA, BI 宿主: 小鼠 Monoclonal C92F3A-5 unconjugated

anti-Heat Shock Protein 70 (HSP70) antibody
HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock Protein 70 (HSP70) antibody (HRP)
HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal HRP

anti-Heat Shock Protein 70 (HSP70) antibody (FITC)
HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal FITC

anti-Heat Shock Protein 70 (HSP70) antibody (Biotin)
HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal Biotin

anti-Heat Shock Protein 70 (HSP70) antibody (PE)
HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal PE

anti-Heat Shock Protein 70 (HSP70) antibody (APC)
HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal APC

anti-Heat Shock Protein 70 (HSP70) antibody (PerCP)
HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal PerCP

anti-Heat Shock Protein 70 (HSP70) antibody (Atto 594)
HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal Atto 594

anti-Heat Shock Protein 70 (HSP70) antibody (Atto 488)
HSP70 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal Atto 488

应用备注
- WB (1:2000)
- optimal dilutions for assays should be determined by the user.
说明

1 μg/ml of ABIN2485875 was sufficient for detection of Drosophila HSP70 using an indirect assay with rabbit anti-rat IgG and goat anti-rabbit IgG:HRP.

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

PBS pH 7.4, 50 % glycerol, 0.1 % sodium azide, Storage buffer may change when conjugated

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

-20°C
Merkling, Overheul, van Mierlo, Arends, Gilissen, van Rij: "The heat shock response restricts virus infection in Drosophila." in: Scientific reports, Vol. 5, pp. 12758, (2015) (PubMed).
抗原

HSP70 (Heat Shock Protein 70 (HSP70))

别名

HSP70

背景

HSP70 genes encode abundant heat-inducible 70- kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50 % identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. For more information visit our HSP70 Scientific Resource Guide at http://www.HSP70.com.

基因ID

48582

NCBI登录号

NP_524927

UniProt

Q9BIS2