P4HB 抗体 (AA 52-63) (Atto 488)
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- 抗原 See all P4HB 抗体
- P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))
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抗原表位
- AA 52-63
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This P4HB antibody is conjugated to Atto 488
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 特异性
- Detects ~48 kDa.
- 交叉反应
- 人
- 纯化方法
- Protein G Purified
- 免疫原
- Synthesized peptide - 12 amino acids long (ADGEDGQDPHSK) corresponding to residues 52 - 63 of the EndoPDI protein
- 克隆位点
- 2E7-7
- 亚型
- IgG2a
- Top Product
- Discover our top product P4HB Primary Antibody
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- 应用备注
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- WB (1:200)
- optimal dilutions for assays should be determined by the user.
- 说明
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1 μg/ml of ABIN2484893 was sufficient for detection of EndoPDI in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti -mouse IgG:HRP as the secondary antibody.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Conjugated antibodies should be stored at 4°C
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- 抗原
- P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))
- 别名
- PDI (P4HB 产品)
- 背景
- Endothelial protein disulfide isomerase (EndoPDI) is a thioredoxin member of the protein disulfide isomerase family of chaperones. This enzyme has been localized to the endoplasmic reticulum in primary endothelial cells, but colocalizes with nucleoli in the nuclei of breast, colon, and renal cancer cells. EndoPDI mRNA and protein expression is induced by hypoxia and exhibits a protective effect on endothelial cells during hypoxia (1).
- 基因ID
- 81567
- NCBI登录号
- NP_001139021
- UniProt
- Q8NBS9
- 途径
- Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism
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