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SOD3 抗体 (Atto 594)

This anti-SOD3 antibody is a 小鼠 单克隆 antibody detecting SOD3 in WB, IHC, ELISA, ICC 和 IF. Suitable for 人.
产品编号 ABIN2484263
发货至: 中国

Quick Overview for SOD3 抗体 (Atto 594) (ABIN2484263)

抗原

See all SOD3 抗体
SOD3 (Superoxide Dismutase 3, Extracellular (SOD3))

适用

  • 60
  • 26
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宿主

  • 57
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  • 3
  • 2
小鼠

克隆类型

  • 62
  • 31
单克隆

标记

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This SOD3 antibody is conjugated to Atto 594

应用范围

  • 77
  • 58
  • 40
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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

克隆位点

4GG11G6
  • 特异性

    Detects extracellular SOD ~35 kDa.

    交叉反应

    豚鼠, 人, 小鼠, 大鼠

    纯化方法

    Protein G Purified

    免疫原

    Human extracellular SOD purified from aortas

    亚型

    IgG1 kappa
  • 应用备注

    • WB (1:1000)
    • IHC (1:100)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.

    说明

    1 μg/ml of ABIN2484263 was sufficient for detection of EC-SOD in 20 μg of human cartilage lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Conjugated antibodies should be stored at 4°C
  • 抗原

    SOD3 (Superoxide Dismutase 3, Extracellular (SOD3))

    别名

    SOD3

    背景

    Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (6). SOD3 can also be distinguished by its heparin-binding capacity (1).

    基因ID

    6649

    NCBI登录号

    NP_003093

    UniProt

    P08294
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