TRPM7 抗体 (AA 1817-1863) (APC)
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Quick Overview for TRPM7 抗体 (AA 1817-1863) (APC) (ABIN2483113)
抗原
See all TRPM7 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1817-1863
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特异性
- Detects ~220 kDa in extracts of cells transiently transfected with TrpM7. No cross-reactivity against TrpM6.
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交叉反应
- 人, 小鼠, 大鼠
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纯化方法
- Protein G Purified
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免疫原
- Fusion protein amino acids 1817-1863 (C- terminus) of mouse TrpM7
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亚型
- IgG1
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应用备注
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- WB (1:1000)
- IHC (1:1000)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
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说明
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1 μg/ml of ABIN2483113 was sufficient for detection of TrpM7 in 10 μg of COS cell lysate transiently transfected with TprM7 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Conjugated antibodies should be stored at 4°C
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- TRPM7 (Transient Receptor Potential Cation Channel, Subfamily M, Member 7 (TRPM7))
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别名
- TRPM7
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背景
- TRPs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants (1, 2).
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基因ID
- 58800
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NCBI登录号
- NP_001157797
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UniProt
- Q923J1
抗原
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