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Piccolo 抗体 (APC)

This anti-Piccolo antibody is a 小鼠 单克隆 antibody detecting Piccolo in WB, ELISA, ICC 和 IF. Suitable for 小鼠.
产品编号 ABIN2482380
发货至: 中国

Quick Overview for Piccolo 抗体 (APC) (ABIN2482380)

抗原

Piccolo (PIC)

适用

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小鼠

宿主

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小鼠

克隆类型

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单克隆

标记

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This Piccolo antibody is conjugated to APC

应用范围

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Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

克隆位点

6H9-B6
  • 特异性

    Detects ~550 kDa. No cross-reactivity to Bassoon.

    交叉反应

    人, 小鼠, 大鼠

    纯化方法

    Protein G Purified

    免疫原

    Full length recombinant protein (GenBank ID: BC001304.1)

    亚型

    IgG2b kappa
  • 应用备注

    • WB (1:1000)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.

    说明

    1 μg/ml of ABIN2482380 was sufficient for detection of Piccolo in 20 μg of rat brain tissue lysate by colorimetric immunoblot analysis using goat IgG:HRP as the secondary antibody.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Conjugated antibodies should be stored at 4°C
  • 抗原

    Piccolo (PIC)

    别名

    Piccolo

    背景

    Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains (1, 2). In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones) (3). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1 (2).

    基因ID

    26875

    NCBI登录号

    NP_001104266

    UniProt

    Q9QYX7
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