CRYAB 抗体 (PerCP)
Quick Overview for CRYAB 抗体 (PerCP) (ABIN2481831)
抗原
See all CRYAB 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Detects ~20 kDa (predicted mol.weight is ~21 kDa). Does not cross-react with αA-crystallin, βL-crystallin, ΒH-crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
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交叉反应
- Cow, 人, 大鼠
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纯化方法
- Protein G Purified
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免疫原
- Native Alpha B Crystallin
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亚型
- IgG1
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anti-Crystallin, alpha B (CRYAB) (AA 1-175) antibody
CRYAB 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Crystallin, alpha B (CRYAB) antibodyCRYAB 适用: 人 WB, IHC, IHC (p), IP, FACS 宿主: 小鼠 Monoclonal 6G5 unconjugated
anti-Crystallin, alpha B (CRYAB) antibodyCRYAB 适用: 人, 大鼠 WB, IHC, StM 宿主: 小鼠 Monoclonal CPTC-CRYAB-1 unconjugated
anti-Crystallin, alpha B (CRYAB) (AA 1-175) antibodyCRYAB 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Crystallin, alpha B (CRYAB) antibodyCRYAB 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Crystallin, alpha B (CRYAB) (AA 1-175) antibodyCRYAB 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 1D11C6E6 unconjugated
anti-Crystallin, alpha B (CRYAB) antibody (HRP)CRYAB 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal HRP
anti-Crystallin, alpha B (CRYAB) antibody (FITC)CRYAB 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal FITC
anti-Crystallin, alpha B (CRYAB) antibody (HRP)CRYAB 适用: 人 WB, ELISA, IHC, IF, ICC 宿主: 小鼠 Monoclonal 3A10-C9 HRP
anti-Crystallin, alpha B (CRYAB) antibody (Biotin)CRYAB 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal Biotin
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应用备注
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- WB (1:2000)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
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说明
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0.5 μg/ml of ABIN2481831 was sufficient for detection of 50 ng purified alpha B crystalline by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Conjugated antibodies should be stored at 4°C
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- CRYAB (Crystallin, alpha B (CRYAB))
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别名
- Alpha B Crystallin
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背景
- The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with HSP25 and HSP27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8). Specifically, alpha-B cystallin is found in many cells and organs outside the lens, and alpha B is overexpressed in several neurological disorders and in cell lines under stress conditions (9).
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基因ID
- 1410
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NCBI登录号
- NP_001876
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UniProt
- P02511
抗原
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