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KDEL 抗体 (Biotin)

This Biotin-conjugated 兔 多克隆 anti-KDEL antibody specifically detects KDEL in WB, IHC, ICC 和 IF. The antibody is reactive with 小鼠, 大鼠 和 人 samples.
产品编号 ABIN2481793
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for KDEL 抗体 (Biotin) (ABIN2481793)

抗原

KDEL (Lys Asp Glu Leu)

适用

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小鼠, 大鼠, 人

宿主

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克隆类型

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多克隆

标记

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This KDEL antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
  • 特异性

    Detects KDEL proteins, GRP94, Grp78, PDI and calreticulin. It may also see ERp57 and ERp29.

    纯化方法

    Protein A Purified

    免疫原

    KDEL containing peptide immunogen
  • 应用备注

    • WB (1:1000)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.

    说明

    A 1:1000 dilution of ABIN2481793 was sufficient for detection of KDEL-containing proteins in 20 μg of HeLa cell lysate by ECL immunoblot analysis using goat anti-mouse IgG as the secondary.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    储存条件

    -20 °C
  • 抗原

    KDEL (Lys Asp Glu Leu)

    别名

    KDEL

    背景

    The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). Grp78 and Grp94 and PDI all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER.
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