HSP90 抗体 (Atto 390)
Quick Overview for HSP90 抗体 (Atto 390) (ABIN2481647)
抗原
See all HSP90 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes 90 kDa. Can isolate complexes of HSP90, Src kinase and cec37.
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交叉反应
- 小鸡, Cow, 人, 小鼠, Pig, 兔, 大鼠
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纯化方法
- Protein G Purified
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免疫原
- Full length protein HSP90 purified from chicken brain
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亚型
- IgG1
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anti-Heat Shock Protein 90 (HSP90) antibody
HSP90 适用: 人 WB, IP, IHC, ELISA, IF, ICC, AA 宿主: 小鼠 Monoclonal H9010 unconjugated
anti-Heat Shock Protein 90 (HSP90) (acLys284), (acLys292) antibodyHSP90 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90 (HSP90) (Lys284), (Lys292) antibodyHSP90 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90 (HSP90) antibody (PE)HSP90 适用: 人 WB, IP, IHC, ELISA, IF, ICC, AA 宿主: 小鼠 Monoclonal H9010 PE
anti-Heat Shock Protein 90 (HSP90) antibody (Atto 390)HSP90 适用: 人 WB, IP, IHC, ELISA, IF, ICC, AA 宿主: 小鼠 Monoclonal H9010 Atto 390
anti-Heat Shock Protein 90 (HSP90) antibodyHSP90 适用: 人 WB, IP, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90 (HSP90) antibody (HRP)HSP90 适用: 人 WB, IP, IHC, ELISA, IF, ICC, AA 宿主: 小鼠 Monoclonal H9010 HRP
anti-Heat Shock Protein 90 (HSP90) antibody (FITC)HSP90 适用: 人 WB, IP, IHC, ELISA, IF, ICC, AA 宿主: 小鼠 Monoclonal H9010 FITC
anti-Heat Shock Protein 90 (HSP90) antibody (Biotin)HSP90 适用: 人 WB, IP, IHC, ELISA, IF, ICC, AA 宿主: 小鼠 Monoclonal H9010 Biotin
anti-Heat Shock Protein 90 (HSP90) antibody (PerCP)HSP90 适用: 人 WB, IP, IHC, ELISA, IF, ICC, AA 宿主: 小鼠 Monoclonal H9010 PerCP
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应用备注
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- WB (1:500)
- IP (5 μg)
- optimal dilutions for assays should be determined by the user.
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说明
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2 μg/ml was sufficient for detection of HSP90α in 20 μg of heat shocked HeLa cell lysate as well as in 100 ng of human HSP90α protein by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Conjugated antibodies should be stored at 4°C
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- HSP90 (Heat Shock Protein 90 (HSP90))
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别名
- HSP90
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背景
- HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (4-7). Despite its label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1-2 % of cytosolic protein). It carries out a number of housekeeping functions - including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90- regulated proteins that have been discovered to date are involved in cell signaling (8-9). The number of proteins now known to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase(6). When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immune-adsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function (10). For more information visit our HSP90 Scientific Resource Guide at http://www.HSP90.ca.
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基因ID
- 9031
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NCBI登录号
- NP_001103255
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UniProt
- P11501
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途径
- M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
抗原
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