Western Blotting (WB), Immunohistochemistry (IHC), ELISA
特异性
Recognizes human mast cell tryptase, both alpha and beta isoforms. Is an excellent marker for mast cells, and does not bind to any other cell type in immunohistology.
纯化方法
Protein G Column
免疫原
Tryptase monoclonal antibody was raised against lug tissue Tryptase (Human).
Tryptase antibody can be used in ELISA, and immunohistochemistry starting at 10 μg/mL. This product requires protein digestion pre-treatment of paraffin sections e.g. pronase. Results may also be obtained with this produst using HIER. Sodium citrate buffer pH 6.0 is recommended for this purpose.
限制
仅限研究用
状态
Liquid
缓冲液
PBS, pH 7.4, 0.09 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
As with all antibodies avoid freeze/thaw cycles. Store undiluted.