MLF1IP 抗体 (Thr78)
Quick Overview for MLF1IP 抗体 (Thr78) (ABIN2470179)
抗原
See all MLF1IP 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Thr78
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特异性
- PThr78
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纯化方法
- Immunoaffinity Chromatography
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免疫原
- MLF1IP antibody was raised against amino acids surrounding Thr78 of MLF1IP (Human).
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anti-MLF1 Interacting Protein (MLF1IP) (pThr78) antibody
MLF1IP 适用: 人 WB, ELISA, IHC, IF, FM 宿主: 兔 Polyclonal unconjugated
anti-MLF1 Interacting Protein (MLF1IP) (N-Term) antibodyMLF1IP 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-MLF1 Interacting Protein (MLF1IP) (Thr78) antibodyMLF1IP 适用: 人, Cow, Chimpanzee, 犬 WB, IHC (p), EIA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- MLF1immunoprecipitation antibody can be used in ELISA, Western Blot, immunohistochemistry starting at 5 μg/mL, and immunoprecipitation
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- As with all antibodies avoid freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Store MLF1IP antibody at -20 °C prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 °C or below.
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- MLF1IP (MLF1 Interacting Protein (MLF1IP))
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别名
- MLF1IP
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背景
- Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel polo-like kinase 1 (Plk1) substrate. Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/anaphase transition. Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry. Mutations of these sites stabilize MLF1IP and inhibit mitotic progression. Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1-dependent MLF1IP degradation. Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block. Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset. MLF1IP is speculated to be a novel tumor suppressor, whose function is required for proper sister-chromatid separation. Loss of MLF1IP function may result in improper segregation of chromosomes and genomic instability, thus promoting tumorigenesis.
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基因ID
- 79682
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UniProt
- Q71F23
抗原
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