Actin 抗体 (N-Term)
Quick Overview for Actin 抗体 (N-Term) (ABIN2465349)
抗原
See all Actin 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- VariantsNP_001604.1 and NP_001135417.1 encode the same protein.
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纯化方法
- Actin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Actin antibody was raised against a 10 amino acid synthetic peptide near the N-Terminus of Actin.
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应用备注
- ELISA: Antibody detection limit dilution 1:4000. Western Blot: Approximately 45 kDa band observed in human duodenum lysates (calculated MW of 42.0 kDa according to NP_001604.1). Recommended concentration: 0.1-0.3 μg/mL. IHC: In paraffin embedded Human and Murine Lung shows staining of the alveolar walls. Recommended concentration, 2-4 g/mL.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 500 μg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20 °C.
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