DEFA1/3 (Internal Region) 抗体
This anti- antibody is a 山羊 多克隆 antibody detecting in ELISA 和 WB. Suitable for 人.
Quick Overview for DEFA1/3 (Internal Region) 抗体 (ABIN2464584)
抗原
DEFA1/3
适用
人
宿主
山羊
克隆类型
多克隆
标记
非结合性
应用范围
ELISA, Western Blotting (WB)
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抗原表位
- Internal Region
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特异性
- This antibody is expected to equally recognize DEFA1 precursor and DEFA3 precursor.
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纯化方法
- DEFA1/3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- DEFA1/3 antibody was raised against a 14 amino acid synthetic peptide near the internal region of DEFA1/3.
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应用备注
- ELISA: Antibody detection limit dilution 1:128000 Western Blot: Approximately 10 kDa band observed in human spleen lysates (calculated MW of 10.2 kDa according to NP_004075.1). Recommended concentration: 1-3 μg/mL. an additional band of unknown identity was also consistently observed at 20 kDa . This band was successfully blocked by incubation with the immunizing peptide Immunohistochemistry: In paraffin embedded thymus shows staining of selected cells. Recommended concentration, 4-6 μg/mL.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 500 μg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20 °C.
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