Neurogranin 抗体 (Internal Region)
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- 抗原 See all Neurogranin (NRGN) 抗体
- Neurogranin (NRGN)
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抗原表位
- Internal Region
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适用
- 人, 小鼠, 大鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Neurogranin antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 纯化方法
- Neurogranin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 免疫原
- Neurogranin antibody was raised against an 11 amino acid synthetic peptide near the internal region of Neurogranin.
- Top Product
- Discover our top product NRGN Primary Antibody
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- 应用备注
- ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 15 kDa band observed in human brain (cerebral cortex and hippocampus), and in mouse brain and rat brain lysates (calculated MW of 7.6 kDa according to NP_006167.1). There is also an additional 16 kDa band. The observed molecular weights correspond to earlier findings in literature with different antibodies (Li et al, J Biol Chem. 1999 Jan 15,274 (3) :1294-300, PMID: 9880498). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human cortex shows cytoplasm staining in the neuronal cell bodies Recommended concentration, 4-6 μg/mL.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 500 μg/mL
- 缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20 °C.
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