Neurogranin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
Neurogranin antibody was raised against an 11 amino acid synthetic peptide near the internal region of Neurogranin.
NRGN
适用: 小鼠, 大鼠
WB, IF (cc), IF (p)
宿主: 兔
Polyclonal
AbBy Fluor® 350
应用备注
ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 15 kDa band observed in human brain (cerebral cortex and hippocampus), and in mouse brain and rat brain lysates (calculated MW of 7.6 kDa according to NP_006167.1). There is also an additional 16 kDa band. The observed molecular weights correspond to earlier findings in literature with different antibodies (Li et al, J Biol Chem. 1999 Jan 15,274 (3) :1294-300, PMID: 9880498). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human cortex shows cytoplasm staining in the neuronal cell bodies Recommended concentration, 4-6 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.