NPT (truncated) 抗体
This anti- antibody is a 小鼠 单克隆 antibody detecting in ELISA 和 WB. Suitable for 人.
Quick Overview for NPT (truncated) 抗体 (ABIN2464088)
抗原
NPT
适用
人
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
ELISA, Western Blotting (WB)
克隆位点
4B4D1
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抗原表位
- truncated
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纯化方法
- Antibody is purified by protein A affinity chromatography.
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免疫原
- Ni-NTA purified truncated recombinant NPT expressed in E. Coli strain BL21 (DE3).
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亚型
- IgG2a
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anti-NPT antibody
适用: 人 ELISA 宿主: 小鼠 Monoclonal 4B4D1 unconjugated
anti-NPT antibody适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 4B4D1 unconjugated
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应用备注
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Western Blot:Dilution 1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test. -
限制
- 仅限研究用
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注意事项
- As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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储存条件
- -20 °C
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储存方法
- NPT monoclonal antibody can be stored at -20 °C, stable for one year.
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- NPT
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背景
- Neomycin phosphotransferase II (nptII) gene is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme. NPTII is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents.
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基因ID
- 11979140
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UniProt
- Q79C30
抗原
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