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IDH3A 抗体

The 兔 多克隆 anti-IDH3A antibody (ABIN2462553) specifically detects IDH3A in WB, ELISA 和 IHC. The antibody is reactive with 人, 大鼠, 小鼠 和 犬 samples.
产品编号 ABIN2462553
发货至: 中国
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Quick Overview for IDH3A 抗体 (ABIN2462553)

抗原

See all IDH3A 抗体
IDH3A (Isocitrate Dehydrogenase 3 (NAD+) alpha (IDH3A))

适用

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人, 大鼠, 小鼠, 犬

宿主

  • 41
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  • 1

克隆类型

  • 43
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多克隆

标记

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This IDH3A antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
  • 纯化方法

    Antibody is purified by protein A chromatography method.

    免疫原

    Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human IDH3A.
  • 应用备注

    IDH3A antibody can be used for detection of IDH3A by ELISA at 1:312500. IDH3A antibody can be used for detection of IDH3A by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

    浓度

    1 mg/mL

    缓冲液

    Antibody is lyophilized in PBS buffer with 2 % sucrose.

    注意事项

    As with any antibody avoid repeat freeze-thaw cycles.

    储存条件

    4 °C/-20 °C

    储存方法

    For short periods of storage (days) store at 4 °C. For longer periods of storage, store IDH3A antibody at -20 °C.
  • 抗原

    IDH3A (Isocitrate Dehydrogenase 3 (NAD+) alpha (IDH3A))

    别名

    IDH3A

    背景

    Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD (+) as the electron acceptor and the other NADP (+). Five isocitrate dehydrogenases have been reported: three NAD (+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP (+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD (+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. IDH3A is the alpha subunit of one isozyme of NAD (+)-dependent isocitrate dehydrogenase.Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD (+) as the electron acceptor and the other NADP (+). Five isocitrate dehydrogenases have been reported: three NAD (+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP (+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD (+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD (+)-dependent isocitrate dehydrogenase.

    分子量

    40 kDa

    基因ID

    3419

    NCBI登录号

    NP_005521

    UniProt

    P50213
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