Actin alpha 2 抗体
This 兔 多克隆 antibody specifically detects Actin alpha 2 in ELISA, WB 和 IHC. It exhibits reactivity toward 小鼠, 大鼠, 犬 和 人.
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产品编号 ABIN2462413
发货至:
中国
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Quick Overview for Actin alpha 2 抗体 (ABIN2462413)
抗原
Actin alpha 2
适用
小鼠, 大鼠, 犬, 人
宿主
兔
克隆类型
多克隆
标记
非结合性
应用范围
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
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纯化方法
- Antibody is purified by protein A chromatography method.
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免疫原
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human Actin alpha 2.
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anti-Actin alpha 2 antibody
适用: 小鼠, 大鼠 ELISA, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Actin alpha 2 antibody can be used for detection of Actin alpha 2 by ELISA at 1:312500. Actin alpha 2 antibody can be used for detection of Actin alpha 2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
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注意事项
- As with any antibody avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C/-20 °C
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储存方法
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store Actin alpha 2 antibody at -20 °C.
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- Actin alpha 2
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背景
- The alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. The two skeletal muscle isoforms of alpha-actinin (ACTN2 and ACTN3) are major structural components of the Z-line involved in anchoring the actin-containing thin filaments. In humans, ACTN2 is expressed in all muscle fibres, while ACTN3 expression is restricted to a subset of type 2 fibres. Murine Actn2 and Actn3 are differentially expressed, spatially and temporally, during embryonic development and, in contrast to humans, alpha-actinin-2 expression does not completely overlap alpha-actinin-3 in postnatal skeletal muscle, suggesting independent function.Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Transcript variants resulting from the use of multiple poly_A sites have been observed.
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分子量
- 98 kDa
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基因ID
- 88
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NCBI登录号
- NP_001094
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UniProt
- P35609
抗原
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