GATAD2B antibody can be used for detection of GATAD2B by ELISA at 1:312500. GATAD2B antibody can be used for detection of GATAD2B by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
限制
仅限研究用
状态
Lyophilized
溶解方式
Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
浓度
1 mg/mL
缓冲液
Antibody is lyophilized in PBS buffer with 2 % sucrose.
注意事项
As with any antibody avoid repeat freeze-thaw cycles.
储存条件
4 °C/-20 °C
储存方法
For short periods of storage (days) store at 4 °C. For longer periods of storage, store GATAD2B antibody at -20 °C.
Hp66beta (GATAD2B), previously identified as a component of the human MeCP1 complex, is a second member of a p66 gene family (hp66alpha and hp66beta). Both proteins interact with MBD2 and MBD3 functionally and biochemically. Both factors function in synergy, since knocking-down p66alpha affects the repressive function of p66beta and vice versa. Furthermore, confocal microscopy shows a nuclear colocalization of hp66alpha with hp66beta and MBD2 in a speckled pattern. These two highly related mammalian p66 proteins display overlapping functions and are involved in methylation dependent transcriptional repression.