E2F3 抗体
Quick Overview for E2F3 抗体 (ABIN2461642)
抗原
See all E2F3 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Antibody is purified by peptide affinity chromatography method.
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免疫原
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human E2F3.
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anti-E2F3 (AA 336-425) antibody
适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 5F7 unconjugated
anti-E2F3 (C-Term) antibody适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-E2F3 (AA 381-465) antibody适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-E2F3 (acLys168) antibody适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-E2F3 (AA 1-108) antibody适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-E2F3 (AA 1-133) antibody适用: 人 ELISA 宿主: 小鼠 Monoclonal 3C11 unconjugated
anti-E2F3 (AA 1-133) antibody适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-E2F3 (C-Term) antibodyVerified 适用: 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-E2F3 (AA 200-300) antibody适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-E2F3 (C-Term) antibody适用: 人, 小鼠, 大鼠, Cow, 犬, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- E2F3 antibody can be used for detection of E2F3 by ELISA at 1:62500. E2F3 antibody can be used for detection of E2F3 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
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注意事项
- As with any antibody avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C/-20 °C
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储存方法
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store E2F3 antibody at -20 °C.
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- E2F3
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背景
- E2F3 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. E2F3 binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner.
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分子量
- 37 kDa, 49 kDa
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基因ID
- 1871
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UniProt
- Q68DT0
抗原
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