ALF 抗体
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北京 101111
Quick Overview for ALF 抗体 (ABIN2460589)
抗原
适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Antibody is purified by peptide affinity chromatography method.
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免疫原
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ALF.
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应用备注
- ALF antibody can be used for detection of ALF by ELISA at 1:312500. ALF antibody can be used for detection of ALF by western blot at 0.4 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
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注意事项
- As with any antibody avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C/-20 °C
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储存方法
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store ALF antibody at -20 °C.
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- ALF
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背景
- The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involves the effects of TFIIA on the interaction between TATA-binding protein (TBP) and DNA. ALF is a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that is able to stabilize the binding of TBP to DNA and may be uniquely important to testis biology.
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分子量
- 50 kDa, 52 kDa, 132 kDa
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基因ID
- 11036
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NCBI登录号
- NP_751946
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UniProt
- Q9UNN4
抗原
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