HMG20A antibody can be used for detection of HMG20A by ELISA at 1:312500. HMG20A antibody can be used for detection of HMG20A by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
限制
仅限研究用
状态
Lyophilized
溶解方式
Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
浓度
1 mg/mL
缓冲液
Antibody is lyophilized in PBS buffer with 2 % sucrose.
注意事项
As with any antibody avoid repeat freeze-thaw cycles.
储存条件
4 °C/-20 °C
储存方法
For short periods of storage (days) store at 4 °C. For longer periods of storage, store HMG20A antibody at -20 °C.
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背景
HMG20A plays a role in neuronal differentiation as chromatin-associated protein. HMG20A acts as inhibitor of HMG20B. HMG20A overcomes the repressive effects of the neuronal silencer REST and induces the activation of neuronal-specific genes. HMG20A involved in the recruitment of the histone methyltransferase MLL and consequent increased methylation of histone H3 lysine 4