NSUN2 抗体
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北京 101111
Quick Overview for NSUN2 抗体 (ABIN2459234)
抗原
See all NSUN2 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Antibody is purified by peptide affinity chromatography method.
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免疫原
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NSUN2.
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anti-NOP2/Sun Domain Family, Member 2 (NSUN2) (AA 508-767) antibody
NSUN2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-NOP2/Sun Domain Family, Member 2 (NSUN2) (AA 432-509) antibodyNSUN2 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-NOP2/Sun Domain Family, Member 2 (NSUN2) (Internal Region) antibodyNSUN2 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-NOP2/Sun Domain Family, Member 2 (NSUN2) (AA 614-663) antibodyNSUN2 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, Pig, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-NOP2/Sun Domain Family, Member 2 (NSUN2) (C-Term) antibodyNSUN2 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-NOP2/Sun Domain Family, Member 2 (NSUN2) (AA 423-451) antibodyNSUN2 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB28701 unconjugated
anti-NOP2/Sun Domain Family, Member 2 (NSUN2) (C-Term) antibodyNSUN2 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 豚鼠, 兔, Pig WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- NSUN2 antibody can be used for detection of NSUN2 by ELISA at 1:62500. NSUN2 antibody can be used for detection of NSUN2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
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注意事项
- As with any antibody avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C/-20 °C
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储存方法
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store NSUN2 antibody at -20 °C.
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- NSUN2 (NOP2/Sun Domain Family, Member 2 (NSUN2))
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别名
- NSUN2
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背景
- Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu (CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 is a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu (CAA).Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu (CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu (CAA) (Brzezicha et al., 2006 [PubMed 17071714]).
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分子量
- 86 kDa
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基因ID
- 54888
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NCBI登录号
- NP_060225
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UniProt
- Q08J23
抗原
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