VRK1 抗体 (N-Term)
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- 抗原 See all VRK1 抗体
- VRK1 (Vaccinia Related Kinase 1 (VRK1))
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抗原表位
- N-Term
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This VRK1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)
- 特异性
- Reacts with human VRK1 protein.
- 交叉反应 （详细）
- Not tested with other species.
- 产品特性
- Mouse monoclonal antibody (5D1) produced in serum-free medium and purified by propriety chromatography under mild conditions (90~98 % pure). Isotype,IgG1 kappa
- 过滤
- Sterile filtered
- 免疫原
- Synthetic peptide corresponding to N-terminus of human VRK1,MPRVKAAQAGRQSSAKRHL-C
- 克隆位点
- 5D1
- 亚型
- IgG2 kappa
- Top Product
- Discover our top product VRK1 Primary Antibody
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anti-Vaccinia Related Kinase 1 (VRK1) (AA 287-396) antibody
VRK1 适用: 人 WB, ELISA, IP, IF, RNAi 宿主: 小鼠 Monoclonal 4F9 unconjugated
anti-Vaccinia Related Kinase 1 (VRK1) (AA 257-396) antibodyVRK1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Vaccinia Related Kinase 1 (VRK1) (AA 1-396) antibodyVRK1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Vaccinia Related Kinase 1 (VRK1) (AA 1-396) antibodyVRK1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Vaccinia Related Kinase 1 (VRK1) antibodyVRK1 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Vaccinia Related Kinase 1 (VRK1) antibodyVRK1 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Vaccinia Related Kinase 1 (VRK1) antibodyVRK1 适用: 人 WB 宿主: 小鼠 Monoclonal 5C1 unconjugated
anti-Vaccinia Related Kinase 1 (VRK1) (Internal Region) antibodyVRK1 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Vaccinia Related Kinase 1 (VRK1) (AA 287-396) antibodyVRK1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Vaccinia Related Kinase 1 (VRK1) (AA 292-329), (C-Term) antibodyVRK1 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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1. Western blotting: 1/200~1/1,000 dilution. Use of highly sensitive chemiluminescence reagents such as Lumi-Light Plus (Roche) or ImmunoStaroR LD (Wako,Tokyo) are recommended.
2. Immunoprecipitation (assay dependent)
3. Immunofluorescence staining: 1/100 dilution
4. Immunohistochemistry (assay dependent)
5. ELISA (assay dependent) - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS with 50 % Glycerol
- 储存液
- Azide free
- 储存条件
- -20 °C
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- 抗原
- VRK1 (Vaccinia Related Kinase 1 (VRK1))
- 别名
- VRK1 (VRK1 产品)
- 别名
- vrk1 antibody, MGC64390 antibody, MGC75762 antibody, VRK1 antibody, PCH1 antibody, PCH1A antibody, 51PK antibody, wu:fe16f06 antibody, zgc:56266 antibody, vaccinia related kinase 1 L homeolog antibody, vaccinia related kinase 1 antibody, Serine/threonine-protein kinase VRK1 antibody, vrk1.L antibody, vrk1 antibody, VRK1 antibody, vrk-1 antibody, Vrk1 antibody
- 背景
- Function, The VRK1 gene encodes serine/threonine kinase VRK1 (Vaccinia-Related Kinase 1, 396 aa, 45.5 kDa) which is involved in Golgi disassembly during the cell cycle following phosphorylation by PLK3 during mitosis, and required to induce Golgi fragmentation. It acts by mediating phosphorylation of a downstream target protein 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. It also phosphorylates casein and histone H3. Phosphorylation of the BANF1 gene product disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Involvement in disease Defects in VRK1 are the cause of pontocerebellar hypoplasia type 1A (PCH1A), also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1A is characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy
- 基因ID
- 7443
- UniProt
- Q99986
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