Rad6 (full length) 抗体
适用: 人, 小鼠, 大鼠, Hamster
WB, IF, IP
宿主: 兔
Polyclonal
unconjugated
(1 reference)-
- 抗原
- Rad6
-
抗原表位
- full length
- 适用
- 人, 小鼠, 大鼠, Hamster
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- 非结合性
- 应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
- 交叉反应 （详细）
- Not tested in other species.
- 纯化方法
- Affinity purified with the immunogen conjugated to agarose
- 过滤
- Sterile filtered
- 免疫原
- Human Rad6B full-length recombinant protein
-
anti-Rad6 antibody
适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
-
1) Western blotting: 1,000~2,000 fold dilution
2) Immunoprecipitation (assay dependent)
3) Immunofluoscent staining (assay dependent)
Other applications have not been tested - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS (x1), 50 % glycerol. Azide and carrier free.
- 储存液
- Azide free
- 储存条件
- -20 °C
- 储存方法
- Upon arrival centrifuge briefly and store at -20 C.
-
-
: "Regulated expression and dynamic changes in subnuclear localization of mammalian Rad18 under normal and genotoxic conditions." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 10, Issue 8, pp. 753-62, (2005) (PubMed).
-
: "Regulated expression and dynamic changes in subnuclear localization of mammalian Rad18 under normal and genotoxic conditions." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 10, Issue 8, pp. 753-62, (2005) (PubMed).
-
- 抗原
- Rad6
- 背景
- Resume of DNA synthesis, which was halted by various physical and chemical factors, was triggered by repair system including Rad6. Rad6 (152a.a, 17.3 kDa) is the ubiqutin-conjugated enzyme E2A, and complex with two Rad18 Molecules, ubiquitin ligase E3, to ubiquitinate PCNA by utilizing poly-ubiquitin chain that HLTF-UBC13 synthesized and they are involved in translesion DNA synthesis (TLS) and template switch (TS). Two isoforms of Rad6, Rad6A and Rad6B differs in only 6 amino acids. They also play an important role in epigenetic transcriptional regulation by catalyzing the monoubiquitination of histone H2B at Lys-120.
- UniProt
- P49459, P63146
-
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