Rad6 (full length) 抗体
This anti- antibody is a 兔 多克隆 antibody detecting in WB, IF 和 IP. Suitable for 人, 小鼠, 大鼠 和 Hamster. This Primary Antibody has been cited in 1 publication.
(1 reference)Quick Overview for Rad6 (full length) 抗体 (ABIN2452101)
抗原
Rad6
适用
人, 小鼠, 大鼠, Hamster
宿主
兔
克隆类型
多克隆
标记
非结合性
应用范围
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
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抗原表位
- full length
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交叉反应 （详细）
- Not tested in other species.
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纯化方法
- Affinity purified with the immunogen conjugated to agarose
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过滤
- Sterile filtered
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免疫原
- Human Rad6B full-length recombinant protein
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anti-Rad6 antibody
适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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1) Western blotting: 1,000~2,000 fold dilution
2) Immunoprecipitation (assay dependent)
3) Immunofluoscent staining (assay dependent)
Other applications have not been tested -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS (x1), 50 % glycerol. Azide and carrier free.
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储存液
- Azide free
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储存条件
- -20 °C
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储存方法
- Upon arrival centrifuge briefly and store at -20 C.
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: "Regulated expression and dynamic changes in subnuclear localization of mammalian Rad18 under normal and genotoxic conditions." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 10, Issue 8, pp. 753-62, (2005) (PubMed).
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: "Regulated expression and dynamic changes in subnuclear localization of mammalian Rad18 under normal and genotoxic conditions." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 10, Issue 8, pp. 753-62, (2005) (PubMed).
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- Rad6
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背景
- Resume of DNA synthesis, which was halted by various physical and chemical factors, was triggered by repair system including Rad6. Rad6 (152a.a, 17.3 kDa) is the ubiqutin-conjugated enzyme E2A, and complex with two Rad18 Molecules, ubiquitin ligase E3, to ubiquitinate PCNA by utilizing poly-ubiquitin chain that HLTF-UBC13 synthesized and they are involved in translesion DNA synthesis (TLS) and template switch (TS). Two isoforms of Rad6, Rad6A and Rad6B differs in only 6 amino acids. They also play an important role in epigenetic transcriptional regulation by catalyzing the monoubiquitination of histone H2B at Lys-120.
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UniProt
- P49459, P63146
抗原
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