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NUP62 抗体 (Phenylalanine-Glycine Repeat)

Cited in 2+ publications. This anti-NUP62 antibody is a 大鼠 单克隆 antibody detecting NUP62 in WB, ELISA, IP, IF 和 ICC. Suitable for 人 和 猴.
产品编号 ABIN2452063
发货至: 中国
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Quick Overview for NUP62 抗体 (Phenylalanine-Glycine Repeat) (ABIN2452063)

抗原

See all NUP62 抗体
NUP62 (Nucleoporin 62kDa (NUP62))

适用

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人, 猴

宿主

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大鼠

克隆类型

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  • 7
单克隆

标记

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This NUP62 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

克隆位点

2A
  • 抗原表位

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    AA 1-179, Phenylalanine-Glycine Repeat

    特异性

    Specific to human (HeLa cells) and simian (Cos cells). The antibody did not react with mouse.

    纯化方法

    Purified from serum-free culture medium of the hybridoma by proprietary chromatography under mild conditions.

    过滤

    Sterile filtered

    免疫原

    Recombinant human Nup62 (amino acids 1-300) (GST-Nup62-His)

    亚型

    IgG1 kappa
  • 应用备注

    1. Western blotting: 1/500 ~1/2,000 dilution
    2. Immunoprecipitation (assay dependent)
    3. Immunofluorescece / Immunocytochemistry: 1/400)
    4. ELISA (assay dependent)
    5. When this antibody was micro-injected into the cytoplasm of the HeLa cells, it accumulates into the nuclear pores as examined by immunofluorescence staining. .

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS (x1), 50 % glycerol. Azide and carrier free.

    储存液

    Azide free

    储存条件

    -20 °C

    储存方法

    Upon arrival centrifuge briefly and store at -20 C.
  • Fukuhara, Sakaguchi, Katahira, Yoneda, Ogino, Tachibana: "Functional analysis of nuclear pore complex protein Nup62/p62 using monoclonal antibodies." in: Hybridoma (2005), Vol. 25, Issue 2, pp. 51-9, (2006) (PubMed).

    Maeshima, Yahata, Sasaki, Nakatomi, Tachibana, Hashikawa, Imamoto, Imamoto: "Cell-cycle-dependent dynamics of nuclear pores: pore-free islands and lamins." in: Journal of cell science, Vol. 119, Issue Pt 21, pp. 4442-51, (2006) (PubMed).

  • 抗原

    NUP62 (Nucleoporin 62kDa (NUP62))

    别名

    Nup62

    背景

    The nuclear pore complex (NPC) regulates cargo transport between the cytoplasm and the nucleus. Nucleoporins are the main components of the NPC in eukaryotic cells. Nup (Nucleoporin) 62 (522 aa, 53 kDa) is a member of the FG-repeat containing nucleoporins and is localized to the NPC central plug. Nup62 associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Predicted to contain about 10 N-acetylglucosamine side chain .

    UniProt

    P37198

    途径

    EGFR Signaling Pathway, SARS-CoV-2 Protein Interactome
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