Influenza Nucleoprotein 抗体 (Influenza A Virus H2N2) (H1N1), (H2N2), (H3N2), (H5N1), (H5N2)
(3 references)Quick Overview for Influenza Nucleoprotein 抗体 (Influenza A Virus H2N2) (H1N1), (H2N2), (H3N2), (H5N1), (H5N2) (ABIN2452039)
抗原
See all Influenza Nucleoprotein (NP) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- H1N1, H2N2, H3N2, H5N1, H5N2
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特异性
- Reacts with NP of all influenza A viruses, including seasonal H2N2, H3N2, and avian H5N1, H5N2 and H1N1 (seasonal, pandemic and swine).
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交叉反应 （详细）
- No cross reactivity with influenza B viruses.
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纯化方法
- Purified by proprietary chromatography procedure under mild conditions.
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纯度
- 90-95 % pure by SDS-PAGE
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过滤
- Sterile filtered
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免疫原
- Human Influenza A Virus (H2N2) Okada strain
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亚型
- IgG2a
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anti-Influenza Nucleoprotein antibody (Influenza A Virus H2N2) (H1N1), (H2N2), (H3N2), (H5N1), (H5N2) (HRP)
NP 适用: Influenza A Virus H2N2 ELISA, WB, IHC, IC 宿主: 小鼠 Monoclonal C43 HRP
anti-Influenza Nucleoprotein antibody (Influenza A Virus H2N2) (AA 2-498)NP 适用: Influenza A Virus H2N2 ELISA 宿主: 小鼠 Monoclonal 9C11 unconjugated
anti-Influenza Nucleoprotein antibody (Influenza B Virus (B/Lee/40))NP 适用: Influenza B Virus ELISA 宿主: 小鼠 Monoclonal 1A2A11 unconjugated
anti-Influenza Nucleoprotein antibody (Influenza A Virus H1N1)NP 适用: Influenza A Virus H1N1 ELISA, WB, PrA 宿主: 小鼠 Monoclonal B248M unconjugated
anti-Influenza Nucleoprotein antibody (Influenza A Virus H1N1)NP 适用: Influenza A Virus H1N1 ELISA, WB, PrA 宿主: 小鼠 Monoclonal B247M unconjugated
anti-Influenza Nucleoprotein antibody (Influenza B Virus)NP 适用: Influenza B Virus ELISA, WB, PrA 宿主: 小鼠 Monoclonal B267M unconjugated
anti-Influenza Nucleoprotein antibody (Influenza A Virus)NP 适用: Influenza A Virus ELISA, WB 宿主: 小鼠 Monoclonal unconjugated
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应用备注
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1) Western blotting: 300~1,000 fold dilution
2) Immuno-precipitation (100 fold dilution
3) Immunofluorescent staining (200 fold dilution
4) Immunohistochemistry (200 fold dilution
4) ELISA (assay dependent) -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS, 50 % glycerol
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储存液
- Azide free
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储存条件
- -20 °C
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: "Development of two types of rapid diagnostic test kits to detect the hemagglutinin or nucleoprotein of the swine-origin pandemic influenza A virus H1N1." in: Clinical and vaccine immunology : CVI, Vol. 18, Issue 3, pp. 494-9, (2011) (PubMed).
: "Maturation efficiency of viral glycoproteins in the ER impacts the production of influenza A virus." in: Virus research, Vol. 136, Issue 1-2, pp. 91-7, (2008) (PubMed).
: "A common neutralizing epitope conserved between the hemagglutinins of influenza A virus H1 and H2 strains." in: Journal of virology, Vol. 67, Issue 5, pp. 2552-8, (1993) (PubMed).
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: "Development of two types of rapid diagnostic test kits to detect the hemagglutinin or nucleoprotein of the swine-origin pandemic influenza A virus H1N1." in: Clinical and vaccine immunology : CVI, Vol. 18, Issue 3, pp. 494-9, (2011) (PubMed).
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- Influenza Nucleoprotein (NP)
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别名
- Influenza A (Nucleoprotein)
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物质类
- Influenza Protein
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背景
- Influenza virus is an RNA virus, which causes influenza, and belongs to the family Orthomyxoviridae. Influenza virus is classified into three different genera, influenzavirus A, B, and C. They all have similar structures and compositions. The virions are 80-100nm in diameter and usually roughly spherical. The outer surface of the virion is made of a viral envelope containing two major glycoproteins, hemagglutinin (HA) and neuraminidase (NA). Influenzavirus A is further classified into subtypes based on the surface glycoproteins, HA and NA. Currently, there are 16 HA and 9 NA subtypes. The central core of the virion contains the viral RNA genome, which is packaged in the form of ribonucleoprotein complexes. Influenza virus nucleoprotein (NP) is a major component of the ribonucleoprotein complex and is abundantly expressed during the course of infection. It is a structural protein, which encapsidates the negative strand viral RNA and is essential for RNA transcription, replication and packaging. NP binds the PB1 and PB2 subunits of the viral RNA polymerase and the matrix protein M1, in addition to its binding to ssRNA. NP is also known to interact with variety of other macromolecules of both viral and cellular origins, and these interactions have been shown to be essential for the viral lifecycle.
抗原
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