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HIV-1 p24 抗体 (Biotin)

This anti-HIV-1 p24 antibody is a 兔 多克隆 antibody detecting HIV-1 p24 in ELISA, WB, IF 和 IP. Suitable for Human Immunodeficiency Virus (HIV). This Primary Antibody has been cited in 4+ publications.
产品编号 ABIN2452028
发货至: 中国

Quick Overview for HIV-1 p24 抗体 (Biotin) (ABIN2452028)

抗原

See all HIV-1 p24 抗体
HIV-1 p24 (Human Immunodeficiency Virus 1 Capsid (HIV-1 p24))

适用

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Human Immunodeficiency Virus (HIV)

宿主

  • 51
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  • 5
  • 1
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克隆类型

  • 53
  • 38
多克隆

标记

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This HIV-1 p24 antibody is conjugated to Biotin

应用范围

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ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
  • 产品特性

    This product is a biotinylated IgG ([biotin]/[IgG] = 8.0) produced from the purified IgG fraction of rabbit anti-p24 serum.

    纯化方法

    Purified

    过滤

    Sterile filtered

    亚型

    IgG
  • 应用备注

    1. Western blotting
    2. Immunoprecipitaiton
    3. Immunofluorescence staining
    4. ELISA
    Not tested in other application

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.9 mg/mL

    缓冲液

    PBS, 50 % glycerol

    储存液

    Azide free

    储存条件

    -20 °C
  • Freed: "HIV-1 gag proteins: diverse functions in the virus life cycle." in: Virology, Vol. 251, Issue 1, pp. 1-15, (1998) (PubMed).

    Saito, Morimoto, Ohara, Takamizawa, Nakata, Shinagawa: "Overproduction, purification, and diagnostic use of the recombinant HIV-1 Gag proteins, the precursor protein p55 and the processed products p17, p24, and p15." in: Microbiology and immunology, Vol. 39, Issue 7, pp. 473-83, (1996) (PubMed).

    Tanaka, Saitoh, Nakata, Shinagawa: "A simple method for overproduction and purification of p24 Gag protein of human immunodeficiency virus type 1." in: Microbiology and immunology, Vol. 36, Issue 8, pp. 823-31, (1993) (PubMed).

    Adachi, Gendelman, Koenig, Folks, Willey, Rabson, Martin: "Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone." in: Journal of virology, Vol. 59, Issue 2, pp. 284-91, (1986) (PubMed).

  • 抗原

    HIV-1 p24 (Human Immunodeficiency Virus 1 Capsid (HIV-1 p24))

    别名

    HIV-1 p24

    物质类

    Viral Protein

    背景

    HIV-1 Gag p24 is a capsid protein that constitutes the core of AIDS virus HIV-1 and is produced by digestion of its precursor Gag p55 by HIV-1 protease. This protein is indispensable to the reproduction of AIDS virus and constitutes an essential element for the AIDS virus particle construction. p24 is used as a marker antigen for observing the patient's condition after treatment, as it indicates the amount of virus in the blood. The product is prepared by immunizing rabbit with recombinant p24 protein which was over-expressed in E. coli with a plasmid carrying the Gag p24 coding region of HIV-1 virus, subtype B, and was highly purified by several steps of chromatography. Using this antiserum in Western blotting, the bands of 24 kD, 55 kD and 41 kD corresponding respectively to HIV-p24 and its precursors p55 and p41 were observed in the extract of the AIDS virus infected cells.
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