适用: Human Immunodeficiency Virus (HIV)
WB, ELISA
宿主: 小鼠
Monoclonal
2H12
unconjugated
应用备注
1. Western blot: 1/1,000~1/3,000 2. Dot blot: 1/3000 3. Immunoprecipitation (assay dependent) 4. ELISA (assay dependent) Other applications have not been tested
限制
仅限研究用
状态
Liquid
缓冲液
0.09 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Upon receipt aliquot and store at -20 C
Arora, Fredericksen, Garcia: "Nef: agent of cell subversion." in: Microbes and infection / Institut Pasteur, Vol. 4, Issue 2, pp. 189-99, (2002) (PubMed).
Fackler, Baur: "Live and let die: Nef functions beyond HIV replication." in: Immunity, Vol. 16, Issue 4, pp. 493-7, (2002) (PubMed).
抗原
HIV-1 Nef
物质类
Viral Protein
背景
HIV-1 Nef (27 kDa) is one of the accessory proteins synthesized in the early stage of AIDS virus reproduction and is abundantly found in infected cells. The name derives from its negative factor thought at the beginning but presently it is remarked as the protein which bears a most distinctive biological characteristic of AIDS virus. The protein interacts directly with the signal transduction protein of the host T cell and works effectively on AIDS infection or on long term survival of the infected cells or induces apoptosis of non-infected cells. It is also involved in the endocytosis and degradation of cell surface receptor proteins such as CD4 and MH4 which are important for AIDS virus infection.