HHV6 gQ1 (AA 3-422) 抗体
适用: Human Herpesvirus 6 (HHV-6)
ELISA, WB, IC, FACS, IF, IP
宿主: 小鼠
Monoclonal
119
unconjugated
(7 references)-
- 抗原
- HHV6 gQ1
- 抗原表位
- AA 3-422
- 适用
- Human Herpesvirus 6 (HHV-6)
- 宿主
- 小鼠
- 克隆类型
- 单克隆
- 应用范围
- ELISA, Western Blotting (WB), Immunochromatography (IC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)
- 特异性
- Reacts with gQ1 of HHV6A and HHV6B. However, this antibody is not recommended for IP of HHV-6B due to conformation specificity.
- 纯化方法
- Produced in serum-free medium and purified by proprietary chromatography procedure under mild conditions.
- 纯度
- 90-95 % pure by SDS-PAGE.
- 过滤
- Sterile filtered
- 免疫原
- His6-tagged recombinant gQ1 of HHV-6A encoding 3-422 amino acids expressed in E. coli.
- 克隆位点
- 119
- 亚型
- IgG
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- 应用备注
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1) Western blotting: 1/500~1/1,000 dilution
2) Immunoprecipitation (assay dependent)
3) Imunofluorescence staining and Immunocytochemistry: 1/100~1/3,200 dilution
4) Flow Cytometry: 1/100
5) ELISA (assay dependent) - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS (x1), 50 % glycerol. Azide and carrier free.
- 储存液
- Azide free
- 储存条件
- -20 °C
- 储存方法
- Upon arrival centrifuge briefly and store at -20 C.
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: "Human herpesvirus 6 glycoprotein complex formation is required for folding and trafficking of the gH/gL/gQ1/gQ2 complex and its cellular receptor binding." in: Journal of virology, Vol. 85, Issue 21, pp. 11121-30, (2011) (PubMed).
: "Human herpesvirus 6 encoded glycoprotein Q1 gene is essential for virus growth." in: Virology, Vol. 407, Issue 2, pp. 360-7, (2010) (PubMed).
: "Human herpesvirus 6 envelope cholesterol is required for virus entry." in: The Journal of general virology, Vol. 87, Issue Pt 2, pp. 277-85, (2006) (PubMed).
: "Discovery of a second form of tripartite complex containing gH-gL of human herpesvirus 6 and observations on CD46." in: Journal of virology, Vol. 78, Issue 9, pp. 4609-16, (2004) (PubMed).
: "Intracellular processing of human herpesvirus 6 glycoproteins Q1 and Q2 into tetrameric complexes expressed on the viral envelope." in: Journal of virology, Vol. 78, Issue 15, pp. 7969-83, (2004) (PubMed).
: "The human herpesvirus 6 U100 gene product is the third component of the gH-gL glycoprotein complex on the viral envelope." in: Journal of virology, Vol. 77, Issue 4, pp. 2452-8, (2003) (PubMed).
: "Human herpesvirus 6 variant A glycoprotein H-glycoprotein L-glycoprotein Q complex associates with human CD46." in: Journal of virology, Vol. 77, Issue 8, pp. 4992-9, (2003) (PubMed).
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: "Human herpesvirus 6 glycoprotein complex formation is required for folding and trafficking of the gH/gL/gQ1/gQ2 complex and its cellular receptor binding." in: Journal of virology, Vol. 85, Issue 21, pp. 11121-30, (2011) (PubMed).
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- 抗原
- HHV6 gQ1
- 物质类
- Viral Protein
- 背景
- Background: Human herpesvirus 6 (HHV-6) is the common collective name for Human herpesvirus 6A (HHV-6A) and Human herpesvirus 6B (HHV-6B). These closely related viruses are two of the nine herpesviruses known to have humans as their primary host. HHV-6A and HHV-6B are double stranded DNA viruses within the betaherpesvirinae subfamily and of the genus Roseolovirus. HHV-6A and HHV-6B infects almost all of the human populations tested. The overall nucleotide sequence identity between HHV- 6A and HHV-6B is 90 % and they are now classified as distinct species. HHV-6A has been described as more neurovirulent, and as such is more frequently found in patients with neuroinflammatory diseases such as multiple sclerosis. HHV-6B primary infection is the cause of the common childhood illness exanthem subitum (also known as roseola infantum or sixth disease). Additionally, HHV-6B reactivation is common in transplant recipients, which can cause several clinical manifestations such as encephalitis, bone marrow suppression and pneumonitis. gQ1 encoded by the U100 gene of HHV6 is glycoprotein, complexes with gH, gL and gQ2 to form HHV6A ligand to CD46 receptor and HHV6B ligand to CD134 receptor. It is expressed in two different forms: an 80- kDa form (gQ1-80K) and a 74- kDa form (gQ1-74K) - only gQ1-80K, but not gQ1-74K, forms the ligand complex with gQ2, gH, and gL. It associates with lipid rafts.
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