Leishmania A2 protein (A2) 抗体
The 小鼠 单克隆 anti-Leishmania A2 protein antibody (Clone C9) (ABIN2443977) specifically detects Leishmania A2 protein in IF, IP 和 WB.
The antibody is reactive with Leishmania donovani (L. donovani) 和 Leishmania samples.
产品编号 ABIN2443977
发货至:
中国
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Quick Overview for Leishmania A2 protein (A2) 抗体 (ABIN2443977)
抗原
Leishmania A2 protein (A2)
适用
Leishmania donovani (L. donovani), Leishmania
宿主
小鼠
克隆类型
单克隆
应用范围
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
克隆位点
C9
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原理
- Leishmania A2 Mouse Monoclonal Antibody (C9)
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序列
- QSVSVGPGPL
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特异性
- A sensitive antibody to Leishmania amastigote-specific A2 protein. This sequence can also be used as tag if cloned with the protein of interest. This sequence is particular to the parasite and therefore the antibody has no cross-reactivity with mammalian proteins. There is no cross-reactivity with other Leishmania species.
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纯化方法
- Protein G purified
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免疫原
- The anti-A2 antibody recognizes only the amastigote form of the A2 protein. The anti-A2 antibody recognizes the sequence QSVGPLSVGP from the parasite, Leishmania donovani.
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亚型
- IgG1
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应用备注
- WB 1:100
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- If reconstituted with deionized water in 100 μL: WB 1:100. Optimal dilution has to be determined by the user.
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缓冲液
- Lyophilized protein G purified in PBS pH 7.4
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
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有效期
- 24 months
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- Leishmania A2 protein (A2)
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别名
- Leishmania A2
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背景
- Leishmania donovani is a protozoa parasite responsible for human visceral leishmaniasis. During their life cycle the parasites exist in 2 forms: as flagellated promastigote within the sandfly vector and as non-flagellated amastigote in the macrophage phagolysosomal compartment of the mammalian host. A2 protein is an amastigote-specific virulence factor that is required for Leishmania donovani parasite survival in a mammalian host.
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UniProt
- A4HZU7
抗原
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