GFP Tag 抗体 (N-Term)
Our Local Distributor
北京 101111
Quick Overview for GFP Tag 抗体 (N-Term) (ABIN2443932)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- N-Term
-
原理
- GFP Tag Mouse Monoclonal Antibody (GF28R)
-
特异性
- The antibody recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP.
-
纯化方法
- Protein G purified
-
免疫原
- GFP from the jellyfish Aequorea victoria N-terminal peptide-KLH conjugates.
-
亚型
- IgG2b
-
-
anti-GFP Tag antibody
适用: 人 WB, IF 宿主: 小鼠 Monoclonal 168AT1211 unconjugated
anti-GFP Tag antibodyWB 宿主: 小鼠 Monoclonal 168AT1211-269-64 unconjugated
anti-GFP Tag antibodyWB, FACS 宿主: 小鼠 Monoclonal 25GB7100 unconjugated
anti-GFP Tag antibodyWB 宿主: 兔 Polyclonal unconjugated
anti-GFP Tag antibody适用: Aequorea victoria ELISA, IF, ICC 宿主: 小鼠 Monoclonal unconjugated
anti-GFP Tag (N-Term) antibody适用: Aequorea victoria WB, ELISA, IHC 宿主: 小鼠 Monoclonal LGB-1 unconjugated
anti-GFP Tag antibodyWB, FACS 宿主: 兔 Monoclonal 25GB7095 unconjugated Recombinant Antibody
-
-
应用备注
- WB 1:1000,IHC 1:500
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- If reconstituted with deionized water in 100 μL: WB 1:1000-3,000, IHC 1:500-2,000. Optimal dilution has to be determined by the user.
-
缓冲液
- Lyophilized protein G purified in PBS pH 7.4
-
储存液
- Without preservative
-
储存条件
- 4 °C,-20 °C,-80 °C
-
储存方法
- Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
-
有效期
- 24 months
-
-
- GFP Tag
-
物质类
- Tag
-
背景
- Epitope tags have application in the labeling, isolation and detection of proteins using immunoblotting, Immunoprecipitation and immunostaining techniques. The green fluorescent protein (GFP) from the jellyfish Aequorea Victoria is involved in the bioluminescence of the jellyfish. When expressed in prokaryotic cells, GFP produces a fluorescent product, making GFP useful in monitoring physiological processes, visualizing protein localization, and detecting transgenic expression.
-
UniProt
- P42212
抗原
-
