Clathrin, Light Chain (N-Term) 抗体
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Quick Overview for Clathrin, Light Chain (N-Term) 抗体 (ABIN2443922)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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原理
- Clathrin Light Chain (CLCa / CLCb) Rabbit Polyclonal Antibody
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特异性
- The antibody recognizes the N-terminal portion of Clathrin Light Chain
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纯化方法
- Immunogen affinity purified
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免疫原
- peptide in N term
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anti-Clathrin, Light Chain (AA 156-173) antibody
适用: 人, 小鼠, 大鼠, 斑马鱼 IHC, WB, ICC, IHC (p) 宿主: 小鼠 Monoclonal 57-4 unconjugated
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应用备注
- IF 1:100,WB 1:1000-1:2000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- If reconstituted with deionized water in 200 μL: IF 1:100 to 1:500, WB 1:1000-1:2000. Optimal dilution has to be determined by the user.
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缓冲液
- Lyophilized immunogen affinity purified in PBS pH 7.4
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
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有效期
- 24 months
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- Clathrin, Light Chain
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别名
- Clathrin Light Chain
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背景
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Clathrin light chain A, Lca
Clathrin-coated vesicles (CCVs) are the major carriers for uptake of nutrients and signalling receptors and for the reformation of synaptic vesicles. Pathogens co-opt the endocytic pathway to gain access into cells. Clathrin is the major component of CCVs and is composed of clathrin-heavy chain and clathrin-light chain (CLC). CLC is a reliable marker of clathrin-coated structures and is also used as a marker of the presynaptic nerve terminal in neurons. Both CLCa and CLCb have alternatively spliced isoforms that are differentially expressed in tissues.
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UniProt
- P09496
抗原
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