Myc Tag 抗体 (C-Term, N-Term)
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北京 101111
Quick Overview for Myc Tag 抗体 (C-Term, N-Term) (ABIN2443920)
抗原
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term, N-Term
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原理
- Myc Tag Mouse Monoclonal Antibody (Myc.A7)
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序列
- EQKLISEEDL
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特异性
- The antibody recognizes the N-terminal or C-terminal Myc-tagged fusion proteins.
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纯化方法
- Protein G purified
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免疫原
- The antibody was raised against EQKLISEEDL (Myc) synthetic peptide conjugated to KLH.
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亚型
- IgG1
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anti-Myc Tag (AA 408-439) antibody
Animal-Free 适用: 标记 WB, ELISA, IF, FACS 宿主: 兔 Monoclonal TUN219-2C1 (9E10) unconjugated Recombinant Antibody
anti-Myc Tag (AA 408-439) antibodyAnimal-Free 适用: 标记 WB, ELISA, IF, FACS 宿主: 小鼠 Monoclonal TUN219-2C1 (9E10) unconjugated Recombinant Antibody
anti-Myc Tag (AA 408-439) antibodyAnimal-Free 适用: 标记 WB, ELISA, IF, FACS 宿主: Human Monoclonal TUN219-2C1 (9E10) unconjugated Recombinant Antibody
anti-Myc Tag (AA 410-419) antibodyWB, IP, ELISA, ICC 宿主: 兔 Monoclonal BLRE01G unconjugated Recombinant Antibody
anti-Myc Tag (C-Term) antibody适用: 人 WB, IP, ELISA, IHC (fro), IHC (p), FACS, ICC, ICFC 宿主: 小鼠 Monoclonal 9E10 unconjugated
anti-Myc Tag (C-Term) antibody (Biotin)适用: 人 WB, IP, ELISA, IHC (fro), IHC (p), FACS, ICC, ICFC 宿主: 小鼠 Monoclonal 9E10 Biotin
anti-Myc Tag (C-Term) antibody (FITC)适用: 人 FACS, ICFC 宿主: 小鼠 Monoclonal 9E10 FITC
anti-Myc Tag antibodyWB, IP, ELISA, IF 宿主: 小鼠 Monoclonal 2G8D5 unconjugated
anti-Myc Tag (AA 408-439) antibody适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC 宿主: 小鼠 Monoclonal 9E10- unconjugated
anti-Myc Tag (AA 408-439) antibody适用: 人 WB, IP, IF, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal 9E10 unconjugated Recombinant Antibody
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应用备注
- WB 1:1000,IHC 1:500
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- If reconstituted with deionized water in 100 μL: WB 1:1000-3,000, IHC 1:500-2,000. Optimal dilution has to be determined by the user.
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缓冲液
- Lyophilized protein G purified in PBS pH 7.4
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
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有效期
- 24 months
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- Myc Tag
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物质类
- Tag
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背景
- Epitope tags have application in the labeling, isolation and detection of proteins using immunoblotting, Immunoprecipitation and immunostaining techniques. Epitope tags can be used, by affinity chromatography, to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. Due its small size the protein's biochemical properties seem not to be affected by the tag protein. The Myc tag is a polypeptide protein corresponding to amino acids 410-419 of human c-Myc (EQKLISEEDL). The anti-Myc tag antibody can be used in a large variety of assays that require recognition by an antibody
抗原
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