EpCAM 抗体
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北京 101111
Quick Overview for EpCAM 抗体 (ABIN2443916)
抗原
See all EpCAM (EPCAM) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Epcam ICD Mouse Monoclonal Antibody (4A7)
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特异性
- The antibody recognizes the intracellular domain of human EpCAM.
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纯化方法
- Protein G purified
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亚型
- IgG1
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anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 40-260) antibody
EPCAM 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 53-150) antibodyEPCAM 适用: 小鼠 WB, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 25-265) antibodyEPCAM 适用: Pig WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) antibodyEPCAM 适用: 人, 小鼠 WB, FACS, IHC, IF 宿主: 兔 Monoclonal EGP40-1555R unconjugated Recombinant Antibody
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 289-314), (Cytoplasmic Domain) antibodyEPCAM 适用: 人, Cat, 犬 IHC 宿主: 小鼠 Monoclonal EGP40-1110 unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 24-265) antibodyEPCAM 适用: 人 WB, FACS, IHC, IP, ICC 宿主: 兔 Monoclonal BLR192J unconjugated Recombinant Antibody
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 264-314) antibodyEPCAM 适用: 人, 小鼠 WB, FACS, IHC, IP, ICC, IF (p) 宿主: 兔 Monoclonal BLR077G unconjugated Recombinant Antibody
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 264-314) antibodyEPCAM 适用: 人, 小鼠 WB, FACS, IHC, IP, ICC, IF (p) 宿主: 兔 Monoclonal BLR077G unconjugated Recombinant Antibody
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 100-224), (Extracellular Domain) antibodyEPCAM 适用: 人, Cat, 犬, 豚鼠 WB, IHC, IF, ELISA 宿主: 小鼠 Monoclonal EGP40-1384 unconjugated
anti-Epithelial Cell Adhesion Molecule (EPCAM) (AA 100-224), (Extracellular Domain) antibodyEPCAM 适用: 人, Cat, 犬 WB, FACS, IHC, IF, ELISA 宿主: 小鼠 Monoclonal EGP40-1373 unconjugated
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应用备注
- WB 1:1000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- If reconstituted with deionized water in 100 μL: WB 1:1000. Optimal dilution has to be determined by the user.
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缓冲液
- Lyophilized protein G purified in PBS pH 7.4
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
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有效期
- 24 months
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- EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))
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背景
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Epithelial Cell Adhesion Molecule Intracellular Domain
Epithelial cell adhesion molecule (EpCAM) is a 40- kDa epithelial transmembrane glycoprotein with an ectodomain, one transmembrane domain, and a cytoplasmic domain of 26 residues. EpCAM is frequently overexpressed in human malignancies, normal stem and progenitor cells, and at lower levels in normal epithelia. In vivo and in vitro studies have shown its intracellular domain (ICD) fragment translocates to nucleus after being cleaved from the EpCAM ectodomain and associates with other molecules to bind DNA resulting in cell proliferation and tumor formation.
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UniProt
- P16422
抗原
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