PRNP 抗体 (pSer43)
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北京 101111
Quick Overview for PRNP 抗体 (pSer43) (ABIN2443909)
抗原
See all PRNP 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer43
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原理
- Phospho Prion Protein (pPrP Ser43) Rabbit Polyclonal Antibody
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序列
- PGGNRYPC
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特异性
- A highly specific and sensitive antibody against the phosphorylation of the Ser43 of the Prion Protein. The antibody reacts with the Ser43 (G[pSer]PGGNRYPC) of the Phosphorylated Prion Protein.
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免疫原
- Phospho-peptide
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anti-Prion Protein (PRNP) (AA 23-230) antibody
PRNP 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Prion Protein (PRNP) (AA 23-230) antibodyPRNP 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 3G10G9 unconjugated
anti-Prion Protein (PRNP) antibodyPRNP 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Prion Protein (PRNP) antibodyPRNP 适用: 人, Hamster WB, ELISA, IHC, IP 宿主: 小鼠 Monoclonal 3F4 unconjugated Recombinant Antibody
anti-Prion Protein (PRNP) (AA 23-230) antibodyPRNP 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Prion Protein (PRNP) (AA 23-64) antibodyPRNP 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Prion Protein (PRNP) antibodyPRNP 适用: 人, 小鼠, 大鼠, Cow, 绵羊 WB, ELISA, IHC, IP, IHC (p) 宿主: 兔 Polyclonal T16-R unconjugated
anti-Prion Protein (PRNP) antibodyPRNP 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Prion Protein (PRNP) antibodyPRNP 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal EM-20 unconjugated
anti-Prion Protein (PRNP) (Prion) antibodyPRNP 适用: 人 WB 宿主: 小鼠 Monoclonal EM-20 unconjugated
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应用备注
- WB 1:100,IP 1:100,IHC 1:200-1:1000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- If reconstituted with deionized water in 50 μL: ELISA (phospho peptide) 1:500,000, E (non-phospho peptide) 1:25,000 WB 1:100, IP 1:100, IHC 1:200-1:1000. Optimal dilution has to be determined by the user
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缓冲液
- Lyophilized serum
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
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有效期
- 24 months
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- PRNP (Prion Protein (PRNP))
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别名
- Prion Protein
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背景
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anti-Phospho Prion Protein, anti-Prion Protein, PrP, ASCR, PrP27-30, PrP33-35C , CD_antigen: CD230
Prion related neurodegenerative diseases, called transmissible spongiform encephalopathies, are observed in many animal species. These diseases involve conversion of normal cellular prion protein (PrPc) into a form that is insoluble and resistant to proteases (PrPSc). The protease resistant form can polymerize into fibrils which accumulate in infected tissues and cause cell death and tissue damage. PrPs have an N-terminal signal sequence and a C-terminal linkage to glycosylphosphatidylinositol anchor. The mature protein is a glycosylated protein that associates with cell membranes. Phosphorylation of PrPC at Ser-43 by Cdk5 promotes proteinase K resistance, prion aggregation, and fibril formation in vitro. In addition, Ser-43 phosphorylation is upregulated in scrapie-infected mouse brain relative to controls. Thus, phosphorylation of Ser-43 may be an important mechanism leading conversion of PrPc to PrPSc and the onset of disease. The antibody was raised against Phospho Prion Protein (pPrP Ser43).
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UniProt
- P04156
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途径
- Transition Metal Ion Homeostasis, Activated T Cell Proliferation
抗原
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