GST-Tag 抗体
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北京 101111
Quick Overview for GST-Tag 抗体 (ABIN2443852)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- GST Tag Mouse Monoclonal Antibody (GST.B6)
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特异性
- The antibody recognizes native and denatured forms of purified GST or GST fusion proteins.
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纯化方法
- Protein G purified
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免疫原
- The antibody was raised against the GST protein.
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亚型
- IgG2a
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应用备注
- WB 1:1000,IHC 1:500
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- If reconstituted with deionized water in 100 μL: WB 1:1000-3,000, IHC 1:500-2,000. Optimal dilution has to be determined by the user.
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缓冲液
- Lyophilized protein G purified in PBS pH 7.4
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
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有效期
- 24 months
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- GST-Tag
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别名
- GST Tag
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物质类
- Tag
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背景
- Glutathione-S-transferases (GSTs) are ubiquitous multifunctional enzymes, which play a key role in cellular detoxification. The GST gene is often used in gene fusion systems for the expression, purification and detection of fusion proteins.
抗原
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