Nucleobindin (full length) 抗体
This 兔 多克隆 antibody specifically detects Nucleobindin in ELISA, ICC, IF, IP 和 WB. It exhibits reactivity toward 人 和 小鼠.
产品编号 ABIN2443849
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Nucleobindin (full length) 抗体 (ABIN2443849)
抗原
Nucleobindin
适用
人, 小鼠
宿主
兔
克隆类型
多克隆
应用范围
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
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抗原表位
- full length
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原理
- Nucleobindin Rabbit Polyclonal Antibody
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特异性
- The antibody recognizes nucleobindin 1 (NucB1)
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纯化方法
- Immunogen affinity purified
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免疫原
- Full length recombinant human nucleobindin protein.
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- If reconstituted with deionized water in 100 μg: WB: 1:1000, IF: 1:150, EM-ICC: 1:50. Optimal dilution has to be determined by the user.
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缓冲液
- Lyophilized immunogen affinity purified in PBS pH 7.4
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
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有效期
- 24 months
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- Nucleobindin
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背景
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NUCB1, NUC, CALNUC
Nucleobindin 1 is a 461 amino acids ubiquitous protein with an N-terminal of 25 amino acids signal peptide responsible for its initial localization to the endoplasmic reticulum (ER). It has also been detected in the cytoplasm and in various subcellular structures such as the nucleus, mitochondria and the Golgi apparatus. Nucleobindin plays a role in a number of cellullar processes such as intracellular signaling, inflammation, autoimmunity, osteogenesis and cancer.
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UniProt
- Q02818
抗原
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