anti-HRP 抗体
This 小鼠 单克隆 antibody specifically detects HRP in IHC. It exhibits reactivity toward Horseradish.
产品编号 ABIN2443813
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
Quick Overview for anti-HRP 抗体 (ABIN2443813)
抗原
HRP
(Horseradish Peroxidase (HRP))
适用
Horseradish
宿主
小鼠
克隆类型
单克隆
应用范围
Immunohistochemistry (IHC)
克隆位点
MAP A6-2
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原理
- Horseradish Peroxidase (HRP) Monoclonal Antibody (MAP A6-2)
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特异性
- A sensitive antibody against horseradish peroxidase (HRP). This antibody was raised against the full horseradish peroxidase (HRP) protein
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纯化方法
- Protein G purified
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免疫原
- HRP protein
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亚型
- IgG1
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anti-Horseradish Peroxidase (HRP) antibody
HRP 适用: Horseradish ELISA, WB, ICC 宿主: 小鼠 Monoclonal HP-03 unconjugated
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应用备注
- IHC 1:30
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- If reconstituted with deionized water in 100 μL: IHC 1:30 - 1:60. Optimal dilution has to be determined by the user.
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缓冲液
- Lyophilized protein G purified in PBS pH 7.4
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
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有效期
- 24 months
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背景
- Horseradish peroxidase (HRP) is a 40 kDa glycoprotein with four lysine residues. In the presence of hydrogen peroxide, HRP will oxidize a substrate to yield a detectable signal useful for techniques such as ELISA and immunohistochemistry. A monoclonal mouse anti-peroxidase antibody is a very useful and reliable reagent for the localization of a number of mouse primary antibodies by peroxidase anti-peroxidase (PAP) immunocytochemistry.
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