AHI1 抗体 (C-Term)
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Quick Overview for AHI1 抗体 (C-Term) (ABIN2443749)
抗原
See all AHI1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term
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原理
- Ahi-1 Mouse Monoclonal Antibody (J5)
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特异性
- A specific and sensitive antibody against Ahi-1. The antibody recognizes the C-terminal of the murine Ahi-1. There is no cross-reactivity with human Ahi-1
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纯化方法
- Protein G purified
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亚型
- IgG1
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- If reconstituted with deionized water in 100μL: WB 1-2 μg/mL, IP 1-2 μg of antibody to 200-600 μg of proteins, IHC 1-5 μg/mL. Optimal dilution has to be determined by the user.
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缓冲液
- Lyophilized protein G purified in PBS pH 7.4
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.
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有效期
- 24 months
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- AHI1 (Abelson Helper Integration Site 1 (AHI1))
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别名
- Ahi-1
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背景
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anti-ahi-1, anti-ahi 1, anti-ahi1, Jouberin , Abelson helper integration site 1 protein , AHI-1
Abelson helper integration site 1 (Ahi-1) is highly expressed in the most primitive normal hematopoietic cells, in brain neurons and in kidney. Ahi-1 is a unique protein with a SH3 domain, multiple SH3 binding sites and a WD40-repeat domain, which are all known to be important mediators of protein-protein interactions. Defects in Ahi-1 are the cause of Joubert syndrome type 3 (JBTS3).
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UniProt
- Q8K3E5
抗原
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