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AHI1 抗体 (C-Term)

The 小鼠 单克隆 anti-AHI1 antibody (Clone J5) (ABIN2443749) specifically detects AHI1 in WB, IHC 和 IP. The antibody is reactive with 小鼠 samples.
产品编号 ABIN2443749
发货至: 中国
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Quick Overview for AHI1 抗体 (C-Term) (ABIN2443749)

抗原

See all AHI1 抗体
AHI1 (Abelson Helper Integration Site 1 (AHI1))

适用

  • 28
  • 5
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
小鼠

宿主

  • 26
  • 3
小鼠

克隆类型

  • 28
  • 1
单克隆

标记

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  • 1
  • 1
  • 1
  • 1
  • 1
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This AHI1 antibody is un-conjugated

应用范围

  • 19
  • 6
  • 4
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  • 3
  • 2
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  • 1
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  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

克隆位点

J5
  • 抗原表位

    • 5
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term

    原理

    Ahi-1 Mouse Monoclonal Antibody (J5)

    特异性

    A specific and sensitive antibody against Ahi-1. The antibody recognizes the C-terminal of the murine Ahi-1. There is no cross-reactivity with human Ahi-1

    纯化方法

    Protein G purified

    亚型

    IgG1
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    If reconstituted with deionized water in 100μL: WB 1-2 μg/mL, IP 1-2 μg of antibody to 200-600 μg of proteins, IHC 1-5 μg/mL. Optimal dilution has to be determined by the user.

    缓冲液

    Lyophilized protein G purified in PBS pH 7.4

    储存液

    Without preservative

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

    有效期

    24 months
  • 抗原

    AHI1 (Abelson Helper Integration Site 1 (AHI1))

    别名

    Ahi-1

    背景

    anti-ahi-1, anti-ahi 1, anti-ahi1, Jouberin , Abelson helper integration site 1 protein , AHI-1

    Abelson helper integration site 1 (Ahi-1) is highly expressed in the most primitive normal hematopoietic cells, in brain neurons and in kidney. Ahi-1 is a unique protein with a SH3 domain, multiple SH3 binding sites and a WD40-repeat domain, which are all known to be important mediators of protein-protein interactions. Defects in Ahi-1 are the cause of Joubert syndrome type 3 (JBTS3).

    UniProt

    Q8K3E5
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