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Myogenin 抗体 (AA 30-224)

The 小鼠 单克隆 anti-Myogenin antibody is suitable to detect Myogenin in samples from 人, 小鼠 和 大鼠. It has been validated for WB, IHC, IHC (p), ICC 和 IHC (fro).
产品编号 ABIN241574
发货至: 中国
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Quick Overview for Myogenin 抗体 (AA 30-224) (ABIN241574)

抗原

See all Myogenin (MYOG) 抗体
Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

适用

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  • 1
人, 小鼠, 大鼠

宿主

  • 74
  • 69
  • 1
小鼠

克隆类型

  • 83
  • 61
单克隆

标记

  • 75
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  • 4
  • 4
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  • 3
  • 3
  • 3
  • 3
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This Myogenin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

5FD
  • 抗原表位

    • 30
    • 16
    • 13
    • 13
    • 10
    • 8
    • 8
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
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    • 1
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    • 1
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    AA 30-224

    特异性

    The 5FD clone has been highly published, users are encouraged to consult the scientific literature for additional application information. In Rh-30, a 34 kD band should be observed. The can be used for gel shift assays.

    纯化方法

    Protein G purified

    免疫原

    A recombinant GST fusion protein corresponding to amino acids 30-224 of rat myogenin.

    Type of Immunogen: Recombinant protein

    亚型

    IgG
  • 应用备注

    Approved: ICC (5 μg/mL), IHC, IHC-Fr (5 - 10 μg/mL), IHC-P (10 μg/mL), WB (2 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

    说明

    Target Species of Antibody: Rat

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS, 0.05 % BSA, 0.05 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-80 °C

    储存方法

    Short term: 4°C
    Long term: -70°C.
  • 抗原

    Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

    别名

    MYOG / Myogenin

    背景

    Name/Gene ID: MYOG

    Synonyms: MYOG, BHLHc3, Myogenin, MYF4, Myogenic factor 4, Myf-4, Myogenin (myogenic factor 4)

    基因ID

    4656

    UniProt

    P15173

    途径

    Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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