NCEH1 抗体 (C-Term)
Quick Overview for NCEH1 抗体 (C-Term) (ABIN238671)
抗原
See all NCEH1 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- AADACL1
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序列
- RTRNSYIKWL DQN
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交叉反应
- Cow, 犬, 人, 小鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-RTRNSYIKWLDQN, from the C Terminus of the protein sequence according to NP_065843.3.
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亚型
- IgG
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anti-Neutral Cholesterol Ester Hydrolase 1 (NCEH1) antibody
NCEH1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Neutral Cholesterol Ester Hydrolase 1 (NCEH1) (AA 224-273) antibodyNCEH1 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 豚鼠, 兔, 斑马鱼, Bat, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Neutral Cholesterol Ester Hydrolase 1 (NCEH1) (AA 90-440) antibodyNCEH1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Neutral Cholesterol Ester Hydrolase 1 (NCEH1) (C-Term) antibodyNCEH1 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 豚鼠, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-Neutral Cholesterol Ester Hydrolase 1 (NCEH1) (Middle Region) antibodyNCEH1 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 豚鼠, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: Preliminary experiments gave an approx 26-28 kDa band in A431 cell lysates and in Human Hippocampus lysates after 1 μg/mL antibody staining. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 h
Peptide ELISA: antibody detection limit dilution 1:1000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the Endoplasmic reticulum of A431 and U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended con
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- NCEH1 (Neutral Cholesterol Ester Hydrolase 1 (NCEH1))
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别名
- AADACL1
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背景
- AADACL1, arylacetamide deacetylase-like 1
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基因ID
- 57552, 320024
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NCBI登录号
- NP_065843, NP_001139748, NP_001139749
抗原
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