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NCEH1 抗体 (C-Term)

This 山羊 多克隆 anti-NCEH1 antibody specifically detects NCEH1 in ELISA, FACS 和 IF. The antibody is reactive with 人 samples.
产品编号 ABIN238671
发货至: 中国
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中国
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Beijing Economic Technological Development Area
Room 801-803
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Quick Overview for NCEH1 抗体 (C-Term) (ABIN238671)

抗原

See all NCEH1 抗体
NCEH1 (Neutral Cholesterol Ester Hydrolase 1 (NCEH1))

适用

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宿主

  • 20
山羊

克隆类型

  • 20
多克隆

标记

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This NCEH1 antibody is un-conjugated

应用范围

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ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

质量等级

Verified
  • 抗原表位

    • 6
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    C-Term

    原理

    AADACL1

    序列

    RTRNSYIKWL DQN

    交叉反应

    Cow, 犬, 人, 小鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-RTRNSYIKWLDQN, from the C Terminus of the protein sequence according to NP_065843.3.

    亚型

    IgG
  • 应用备注

    Western Blot: Preliminary experiments gave an approx 26-28 kDa band in A431 cell lysates and in Human Hippocampus lysates after 1 μg/mL antibody staining. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 h

    Peptide ELISA: antibody detection limit dilution 1:1000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the Endoplasmic reticulum of A431 and U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended con

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    NCEH1 (Neutral Cholesterol Ester Hydrolase 1 (NCEH1))

    别名

    AADACL1

    背景

    AADACL1, arylacetamide deacetylase-like 1

    基因ID

    57552, 320024

    NCBI登录号

    NP_065843, NP_001139748, NP_001139749
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