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Arylsulfatase A 抗体 (Internal Region)

Cited in 4+ publications. This anti-Arylsulfatase A antibody is a 山羊 多克隆 antibody detecting Arylsulfatase A in WB, IHC, ELISA, IF 和 FACS. Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN238536
发货至: 中国
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Quick Overview for Arylsulfatase A 抗体 (Internal Region) (ABIN238536)

抗原

See all Arylsulfatase A (ARSA) 抗体
Arylsulfatase A (ARSA)

适用

  • 49
  • 34
  • 33
  • 4
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  • 1
  • 1
人, 小鼠, 大鼠

宿主

  • 49
  • 15
山羊

克隆类型

  • 51
  • 13
多克隆

标记

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  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
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  • 1
  • 1
  • 1
  • 1
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This Arylsulfatase A antibody is un-conjugated

应用范围

  • 49
  • 17
  • 16
  • 13
  • 13
  • 8
  • 6
  • 6
  • 6
  • 5
  • 3
  • 1
  • 1
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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

质量等级

Verified
  • 抗原表位

    • 15
    • 12
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region

    原理

    Arylsulfatase A

    序列

    YDLSKDPGEN YN

    交叉反应

    人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-YDLSKDPGENYN, from the internal region of the protein sequence according to NP_000478.2.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Brain (Cortex) and Testis. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 60-65Da band observed in Mouse and Rat Testis lysates (calculated MW of 53.7 kDa according to Mouse NP_033843.2 and Rat NP_001030105.2,). This molecular weight is routinely observed by other sources and was successfully blocked by inc

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the Golgi apparatus of HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10u

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Kanbe, Kamijo, Nakajima, Tanaka, Sugiyama, Wang, Fang, Hara, Gonzalez, Aoyama: "Chronic ethanol consumption decreases serum sulfatide levels by suppressing hepatic cerebroside sulfotransferase expression in mice." in: Archives of toxicology, Vol. 88, Issue 2, pp. 367-79, (2014) (PubMed).

    Kimura, Nakajima, Kamijo, Tanaka, Wang, Hara, Sugiyama, Tanaka, Gonzalez, Aoyama: "Hepatic Cerebroside Sulfotransferase Is Induced by PPAR? Activation in Mice." in: PPAR research, Vol. 2012, pp. 174932, (2012) (PubMed).

    Kiebish, Young, Lehman, Han: "Chronic caloric restriction attenuates a loss of sulfatide content in PGC-1?-/- mouse cortex: a potential lipidomic role of PGC-1? in neurodegeneration." in: Journal of lipid research, Vol. 53, Issue 2, pp. 273-81, (2012) (PubMed).

    Zhang, Nakajima, Kamijo, Li, Hu, Kannagi, Kyogashima, Aoyama, Hara: "Acute kidney injury induced by protein-overload nephropathy down-regulates gene expression of hepatic cerebroside sulfotransferase in mice, resulting in reduction of liver and serum sulfatides." in: Biochemical and biophysical research communications, Vol. 390, Issue 4, pp. 1382-8, (2009) (PubMed).

  • 抗原

    Arylsulfatase A (ARSA)

    别名

    ARSA

    背景

    ARSA, arylsulfatase A, MLD, cerebroside-sulfatase, cerebroside 3-sulfatase

    基因ID

    410, 11883, 315222

    NCBI登录号

    NP_000478
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